Lobão-Soares Bruno, Walz Roger, Carlotti Carlos Gilberto, Sakamoto Américo Ceiki, Calvo Fabrício, Terzian Ana Luiza Bernardes, da Silva Juliana Almeida, Wichert-Ana Lauro, Coimbra Norberto Cysne, Bianchin Marino Muxfeldt
Laboratório de Neuroanatomia & Neuropsicobiologia, Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (USP), Av. dos Bandeirantes, 3900, Ribeirão Preto, SP 14049-900, Brasil.
Behav Brain Res. 2007 Oct 1;183(1):87-94. doi: 10.1016/j.bbr.2007.05.027. Epub 2007 May 26.
The cellular prion protein (PrP(C)) is a sialoglycoprotein involved in neuroplasticity processes and synaptic transmission. This study investigated behavioural responses (balance in the rota-rod test at 24 rpm, motility in the open-field test, anxiety in the elevated plus-maze test) in Zurich developed wild-type adult mice (WT, controls of normal PrP(C) expression), in knockout (KO) mice (Prnp(0/0), with no PrP(C) expression), and in PrP(C) overexpressing Tg-20 mice. After 8 min in the rota-rod test, Tg-20 animals presented significantly fewer falls (1.08+/-1.56 falls) than both WT (7.27+/-4.36) and KO (7.6+/-6.15) mice (p<0.01). In the open field test, Tg-20 animals showed significantly increased motility [rearing=23.4+/-7.85, crossing=97.30+/-32.11) when compared with KO mice (rearing=5.45+/-3.69 and crossing=59.73+/-15.43) or WT mice (rearing=6.5+/-20.23 and crossing=45.18+/-20.33) (p<0.01). In the elevated plus-maze test, Tg-20 mice showed less anxiety (head projections=7.3+/-1.62) when compared with WT animals (3.38+/-0.67) (p<0.05). Moreover, KO mice spent more time in the centre of the plus maze (37.80+/-5.57 s) than did WT mice (22.57+/-3.82) (p<0.05). PrP(C) overexpressing mice evoked increased motility, less anxiety, and increased equilibrium when compared with WT control animals in the behavioural protocols used. KO animals also tended to evoke fewer anxiety-related responses in the elevated plus-maze test. These findings indicate that the levels of PrP(C) in adult life are associated with possible changes in motility, anxiety, and equilibrium.
细胞朊蛋白（PrP(C)）是一种参与神经可塑性过程和突触传递的唾液酸糖蛋白。本研究调查了苏黎世培育的野生型成年小鼠（WT，正常PrP(C)表达的对照组）、基因敲除（KO）小鼠（Prnp(0/0)，无PrP(C)表达）和PrP(C)过表达的Tg-20小鼠的行为反应（24转/分钟的转棒试验中的平衡能力、旷场试验中的运动能力、高架十字迷宫试验中的焦虑水平）。在转棒试验8分钟后，Tg-20动物的跌倒次数（1.08±1.56次跌倒）明显少于WT小鼠（7.27±4.36次）和KO小鼠（7.6±6.15次）（p<0.01）。在旷场试验中，与KO小鼠（直立次数=5.45±3.69次，穿越次数=59.73±15.43次）或WT小鼠（直立次数=6.5±20.23次，穿越次数=45.18±20.33次）相比，Tg-20动物的运动能力显著增强（直立次数=23.4±7.85次，穿越次数=97.30±32.11次）（p<0.01）。在高架十字迷宫试验中，与WT动物（3.38±0.67次）相比，Tg-20小鼠的焦虑水平较低（头部探出次数=7.3±1.62次）（p<0.05）。此外，KO小鼠在十字迷宫中央停留的时间（37.80±5.57秒）比WT小鼠（22.57±3.
