Carnell N E, Feng P, Kim U J, Wilber J F
Division of Endocrinology, University of Maryland School of Medicine, Baltimore 21201.
Neuropeptides. 1992 Aug;22(4):209-12. doi: 10.1016/0143-4179(92)90047-z.
PreproTRH mRNA has been identified in rat cardiac tissues by Northern analyses and RNase protection assays with a specific rat 32P-TRH cRNA probe. Densitometric analyses revealed that atrial ppTRH mRNA concentrations were approximately five-fold greater than those of the ventricles. TRH concentrations (RIA), by contrast, were two-fold higher in ventricles. These data suggest that TRH and TRH mRNA are present in the rat heart, but their concentrations are dissociated, possibly because of differential post-transcriptional or post-translational processing. TRH is postulated to play an autocrine or paracrine role in cardiac physiology.
通过Northern分析和用特异性大鼠32P-TRH cRNA探针进行的核糖核酸酶保护试验，已在大鼠心脏组织中鉴定出前促甲状腺激素释放激素（PreproTRH）mRNA。光密度分析显示，心房中ppTRH mRNA浓度约为心室的五倍。相比之下，心室中的促甲状腺激素释放激素（TRH）浓度（放射免疫分析）则高出两倍。这些数据表明，TRH和TRH mRNA存在于大鼠心脏中，但它们的浓度是分离的，这可能是由于转录后或翻译后加工的差异所致。据推测，TRH在心脏生理学中发挥自分泌或旁分泌作用。
