Stefanov R, Angelova M, Stefanova T, Subev M, Dolashka P, Voelter W, Zachariev Z
Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Andrologia. 2004 Apr;36(2):51-6. doi: 10.1111/j.1439-0272.2004.00604.x.
In this study we determined the effect of reactive oxygen species (ROS) generation during incubation in media at 39 degrees C on ram spermatozoa and the protection by exogenously added antioxidant enzyme, superoxide dismutase (SOD). A novel Cu/Zn-SOD, isolated from the fungal strain Humicola lutea 103 (HLSOD), was used. Our results point out that the levels of both, superoxide anion radicals (*O2-) and H2O2, increase approximately 8-10- and 2-3-fold, respectively, during incubation of spermatozoa. Enhanced ROS generation coincided with reduction of motility, independently of the type of diluted medium. Addition of HLSOD (30, 60 and 120 U ml(-1) sperm) improved sperm functions, maintaining almost initial percentages of motile spermatozoa and increasing the values of mean cytochemical coefficient. At the same time, a significant diminution of *O2- and H2O2 content in the presence of antioxidant enzyme was established. The results suggest that HLSOD is an effective *O2- scavenger in semen that leads to protection of sperm functions.
在本研究中，我们测定了39℃下在培养基中孵育期间活性氧（ROS）生成对公羊精子的影响以及外源性添加抗氧化酶超氧化物歧化酶（SOD）的保护作用。使用了从真菌菌株黄腐质霉103（HLSOD）中分离出的一种新型铜/锌超氧化物歧化酶。我们的结果指出，在精子孵育期间，超氧阴离子自由基（*O₂⁻）和过氧化氢（H₂O₂）的水平分别增加了约8 - 10倍和2 - 3倍。ROS生成增强与活力降低同时出现，且与稀释培养基的类型无关。添加HLSOD（30、60和120 U/ml精子）可改善精子功能，维持几乎初始的活动精子百分比，并增加平均细胞化学系数值。同时，在存在抗氧化酶的情况下，O₂⁻和H₂O₂含量显著降低。结果表明，HLSOD是精液中一种有效的O₂⁻清除剂，可保护精子功能。
