Büyükbaba-Boral Ozden, Gönüllü Nevriye, Küçükbasmaci Omer, Anğ-Küçüker Mine, Anğ Ozdem
Department of Microbiology and Clinical Microbiology, Istanbul Faculty of Medicine, Istanbul, Turkey.
Chemotherapy. 2004 Apr;50(1):35-9. doi: 10.1159/000077283.
The influence of urine on the in vitro activities of various antibiotics used in the therapy of urinary tract infections was assessed by the microbroth dilution method in this study.
Thirty Escherichia coli strains were used: 10 E. coli strains susceptible to ampicillin, 10 strains resistant to ampicillin and ampicillin+sulbactam and ten extended spectrum beta-lactamase producer strains. The minimum inhibitory concentrations (MICs) of ampicillin, ampicillin + sulbactam, cephalothin, cefuroxime, ceftriaxone, amikacin, ciprofloxacin, sulfamethoxazole and trimethoprim were performed parallel in Mueller-Hinton broth and human urine by the microbroth dilution method.
The MIC(90) of all antibiotics except cephalothin were higher in the urine. MICs performed in the urine were found significantly higher than those performed in broth.
This study demonstrated that MICs of antibiotics are influenced by the human urine and that MICs of some antibiotics used in the treatment of urinary tract infections may be overestimated by the standard antibiotic testing methods.
本研究采用微量肉汤稀释法评估尿液对用于治疗尿路感染的各种抗生素体外活性的影响。
使用了30株大肠杆菌菌株：10株对氨苄西林敏感的大肠杆菌菌株、10株对氨苄西林和氨苄西林+舒巴坦耐药的菌株以及10株产超广谱β-内酰胺酶的菌株。通过微量肉汤稀释法，在 Mueller-Hinton 肉汤和人尿中平行测定氨苄西林、氨苄西林+舒巴坦、头孢噻吩、头孢呋辛、头孢曲松、阿米卡星、环丙沙星、磺胺甲恶唑和甲氧苄啶的最低抑菌浓度（MIC）。
除头孢噻吩外，所有抗生素在尿液中的MIC（90）均较高。发现在尿液中测定的MIC显著高于在肉汤中测定的MIC。
本研究表明抗生素的MIC受人体尿液影响，并且标准抗生素检测方法可能高估了一些用于治疗尿路感染的抗生素的MIC。
