活体亲属供肝移植中移植物的壁面剪应力与肝内白细胞

Wall shear stress and intrahepatic leukocytes of graft in living related donor liver transplantation.

作者信息

Sato Yoshinobu, Watanabe Hisami, Ichida Takafumi, Yamamoto Satoshi, Nakatsuka Hideki, Oya Hiroshi, Kameyama Hiroshi, Watanabe Takaoki, Shimamura Kazuhiko, Abo Toru, Hatakeyama Katsuyoshi

机构信息

Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.

出版信息

Hepatogastroenterology. 2004 Mar-Apr;51(56):329-33.

PMID:15086151

Abstract

BACKGROUND/AIMS: We investigated the influence of HTK solution against natural killer T cells and thymic T cells in liver graft before and after perfusion in adult living related donor liver transplantation.

METHODOLOGY

Graft samples were obtained before liver resection, after perfusion, and one hour after liver transplantation. Flowcytometry analysis was conducted using several human natural killer markers; CD16, CD56, CD57, and CD161.

RESULTS

Natural killer T cells existed prominently in the liver leukocytes compared with their presence in peripheral blood lymphocytes, and the difference was significant. CD56+ T and CD161+ T cells, in comparison with CD16+ T cells and CD57+ T cells, were especially numerous in the liver. The proportion of CD56+ T and CD161+ T cells increased in the graft immediately after perfusion with HTK solution. However, CD16+ T cells and CD57+ T cells decreased in the graft immediately after perfusion and reperfusion of portal blood flow. Thymus-derived cells also decreased significantly after perfusion. The proportion of CD56+ T cells among CD3+ cells showed a significant increase immediately after perfusion. All types of natural killer cells in the graft immediately increased after perfusion by HTK solution and reperfusion of portal blood flow. Compared with CD57+ NKT cells, CD56+ NKT cells showed a significant tendency to stay in the liver graft against the perfusion. CD57+ NKT cells tended to wash out from the liver into the systemic circulation. Moreover, thymus-derived T cells showed the strongest tendency to wash out from the liver graft.

CONCLUSIONS

CD56+ NKT cells and natural killer cells are more involved in local immunity, whereas thymus-derived cells and CD57+ NKT cells are involved in regulation of systemic immunity. Alloimmunity between local and systemic systems may be affected by the dynamic changes in hepatic circulation associated with living related donor liver transplantation.

摘要

背景/目的：我们研究了在成人活体亲属供肝移植中，HTK液对灌注前后肝移植物中自然杀伤T细胞和胸腺T细胞的影响。

方法

在肝切除前、灌注后以及肝移植后1小时获取移植物样本。使用几种人类自然杀伤标志物进行流式细胞术分析；CD16、CD56、CD57和CD161。

结果

与外周血淋巴细胞相比，自然杀伤T细胞在肝白细胞中显著存在，差异有统计学意义。与CD16+T细胞和CD57+T细胞相比，CD56+T细胞和CD161+T细胞在肝脏中尤其丰富。在用HTK液灌注后，移植物中CD56+T细胞和CD161+T细胞的比例立即增加。然而，在门静脉血流灌注和再灌注后，移植物中CD16+T细胞和CD57+T细胞立即减少。灌注后胸腺来源的细胞也显著减少。灌注后，CD3+细胞中CD56+T细胞的比例立即显著增加。在用HTK液灌注和门静脉血流再灌注后，移植物中所有类型的自然杀伤细胞立即增加。与CD57+自然杀伤T细胞相比，CD56+自然杀伤T细胞在灌注时更倾向于留在肝移植物中。CD57+自然杀伤T细胞倾向于从肝脏冲刷到体循环中。此外，胸腺来源的T细胞从肝移植物中冲刷出去的倾向最强。