Ahn W S, Bae S M, Lee J M, Namkoong S E, Yoo J Y, Seo Y-S, Nam S L, Cho Y-L, Nam K H, Kim C K, Kim Y-W
Department of Obstetrics and Gynecology, Catholic Research Institutes of Medical Science, Seoul, Korea.
Int J Gynecol Cancer. 2004 Mar-Apr;14(2):322-32. doi: 10.1111/j.1048-891x.2004.014217.x.
To evaluate anti-tumor effects of recombinant adenovirus p53, time-course p53, E6 expression, and cell growth inhibition were investigated in vitro and in vivo using cervical cancer cell lines such as CaSki, SiHa, HeLa, HeLaS3, C33A, and HT3. The cell growth inhibition was studied via cell count assay, MTT assay and neutral red assay. After transfecting AdCMVp53 into SiHa cells-xenografted nude mice, the transduction efficiency and anti-tumor effect were investigated for a month. The results showed that adenoviral p53 expression induced significant growth suppression on the cancer cells, in which E6 transcript was strongly repressed, and that the expression of p53 and E6 were remarkably dependent on each cell type. The transduction efficiency was highly maintained in vivo as well as in vitro, and the size of tumor was remarkably decreased in comparison with AdCMVLacZ control. The results suggest that the adenovirus-mediated p53 gene transfection was done very effectively in vitro and in vivo experiment, and the cell growth was suppressed via p53-dependent apoptotic cell death, and that the anti-tumor effect could be related to E6 and p53 expression pattern.
为评估重组腺病毒p53的抗肿瘤作用，利用CaSki、SiHa、HeLa、HeLaS3、C33A和HT3等宫颈癌细胞系在体外和体内研究了p53的时间进程、E6表达及细胞生长抑制情况。通过细胞计数法、MTT法和中性红法研究细胞生长抑制情况。将AdCMVp53转染到异种移植裸鼠的SiHa细胞中后，对转导效率和抗肿瘤作用进行了为期一个月的研究。结果显示，腺病毒p53表达对癌细胞有显著的生长抑制作用，其中E6转录本被强烈抑制，且p53和E6的表达明显依赖于每种细胞类型。转导效率在体内和体外均能高度维持，与AdCMVLacZ对照相比，肿瘤大小显著减小。结果表明，腺病毒介导的p53基因转染在体外和体内实验中均能非常有效地进行，细胞生长通过p53依赖性凋亡细胞死亡受到抑制，且抗肿瘤作用可能与E6和p53表达模式有关。
