Ozturk B, Ozer F, Belli S
Department of Restorative Dentistry, Selcuk University, Faculty of Denstistry, Konya, Turkey.
Int Endod J. 2004 May;37(5):297-306. doi: 10.1111/j.0143-2885.2004.00790.x.
To compare in vitro the sealing properties of five different dentine adhesive materials (Prime&Bond NT (PBNT); Prompt L-Pop (PLP); Clearfil SE Bond (CSEB); Scotchbond Multi Purpose Plus (SMPP); EBS-Multi (EBSM)) inside the pulp chamber.
Seventy-five recently extracted human molar teeth were used. The roof of the pulp chambers and roots were removed under water cooling. Pulp tissue was removed, and the canal orifices were sealed. The pulp chambers were then treated with 5% sodium hypochlorite (NaOCl) for 1 min. The teeth were randomly divided into five groups of 15 teeth each. Adhesive systems were applied to the pulp chamber walls according to the manufacturers' instructions. The samples were connected to Plexiglass plates, and a fluid filtration method was used for quantitative evaluation of leakage. Measurements of fluid movement were made at 2-min intervals for 8 min. The quality of seal of each specimen was measured immediately, after 24 h, 1 week and 1 month. The data were statistically analysed by repeated-measurements multivariate anova, Friedman test, Wilcoxon signed rank test, Kruskal-Wallis of one-way anova and Mann-Whitney U-tests. The pulp chamber wall with and without NaOCl and resin-dentine interfaces of specimens were observed under a scanning electron microscope (SEM).
The leakage values of the materials were significantly different at different measurement periods. In all groups, leakage values decreased with time (P < 0.05). PBNT and PLP had the least leakage during immediate measurements (P < 0.05). After 1 month, leakage of all adhesive systems was not significantly different (P < 0.05). SEM observation of pulp chamber walls demonstrated that the irregular dentine surface without smear layer was present in the nontreated group. However, NaOCl application removed the collagen fibrils leaving the dentine surface smooth. At resin-dentine interfaces of specimens, no hybridization zone was observed.
None of the materials had created a perfect seal to the pulp chamber walls. PBNT and PLP had better sealing over the short term, but over the long term, there were no differences between the materials.
在体外比较五种不同牙本质粘结材料（Prime&Bond NT（PBNT）；Prompt L-Pop（PLP）；Clearfil SE Bond（CSEB）；Scotchbond Multi Purpose Plus（SMPP）；EBS-Multi（EBSM））在髓腔内的封闭性能。
使用75颗近期拔除的人类磨牙。在水冷条件下去除髓室顶和牙根。去除牙髓组织，并封闭根管口。然后用5%次氯酸钠（NaOCl）处理髓腔1分钟。将牙齿随机分为五组，每组15颗。按照制造商的说明将粘结系统应用于髓腔壁。将样本连接到有机玻璃板上，并使用流体过滤法对渗漏进行定量评估。每隔2分钟测量一次流体移动，共测量8分钟。在即刻、24小时、1周和1个月后立即测量每个标本的封闭质量。数据采用重复测量多因素方差分析、Friedman检验、Wilcoxon符号秩检验、单向方差分析的Kruskal-Wallis检验和Mann-Whitney U检验进行统计学分析。在扫描电子显微镜（SEM）下观察标本有无NaOCl处理的髓腔壁以及树脂-牙本质界面。
在不同测量时期，材料的渗漏值有显著差异。在所有组中，渗漏值随时间下降（P<0.05）。在即刻测量时，PBNT和PLP的渗漏最少（P<0.05）。1个月后，所有粘结系统的渗漏无显著差异（P<0.05）。对髓腔壁的SEM观察表明，未处理组存在无玷污层的不规则牙本质表面。然而，应用NaOCl去除了胶原纤维，使牙本质表面光滑。在标本的树脂-牙本质界面，未观察到混合层。
没有一种材料能对髓腔壁形成完美的封闭。PBNT和PLP在短期内具有较好的封闭性，但从长期来看，材料之间没有差异。
