Raposo F, Borja R, Sánchez E, Martín M A, Martín A
Instituto de la Grasa y sus Derivados (CSIC), Avda. Padre García Tejero 4, Sevilla E-41012, Spain.
Water Res. 2004 Apr;38(8):2017-26. doi: 10.1016/j.watres.2004.01.007.
A lab-scale study was conducted on the mesophilic anaerobic digestion of two-phase olive mill effluents constituted by the mixture of the wash waters derived from the initial cleansing of the olives and those obtained in the washing and purification of virgin olive oil. The digestion was conducted in two continuously stirred tank reactors, one with biomass immobilized on Bentonite (reactor B) and other with suspended biomass used as control (reactor C). The reactors B and C operated satisfactorily between hydraulic retention times of 25.0 and 4.0 days and 25.0 and 5.0 days, respectively. Total chemical oxygen demand (TCOD) efficiencies in the ranges of 88.8-72.1% and 87.9-71.2% were achieved in the reactors with immobilized and suspended biomass, respectively, at organic loading rates of between 0.86 and 5.38 g TCOD/ld and 0.86-4.30 g TCOD/ld, respectively. On comparing both reactors for the same OLRs applied, it was observed that the reactor with support was always more efficient and stable showing higher TCOD, SCOD removal efficiencies and lower VFA/alkalinity ratio values than those found in the control reactor. A mass (TCOD) balance around the reactors allowed the methane yield coefficient, Y(G/S), to be obtained, which gave values of 0.31 and 0.30l CH(4)/g TCOD(removed) for reactors B and C, respectively. The cell maintenance coefficients,k(m), obtained by means of this balance were found to be 0.0024 and 0.0036 g TCOD(removed)/g VSSd, respectively. The volumetric methane production rates correlated with the biodegradable TCOD concentration through an equation of the Michaelis-Menten type for the two reactors studied. This proposed model predicted the behavior of the reactors very accurately showing deviations lower than 10% between the experimental and theoretical values of methane production rates.
进行了一项实验室规模的研究，以考察两相橄榄果渣废水的中温厌氧消化情况。该废水由橄榄初次清洗产生的洗涤水与初榨橄榄油洗涤和提纯过程中获得的洗涤水混合而成。消化过程在两个连续搅拌槽式反应器中进行，一个反应器中生物质固定在膨润土上（反应器B），另一个反应器中使用悬浮生物质作为对照（反应器C）。反应器B和C分别在水力停留时间为25.0至4.0天和25.0至5.0天的条件下运行良好。在固定化生物质和悬浮生物质的反应器中，当有机负荷率分别为0.86至5.38 g TCOD/ld和0.86 - 4.30 g TCOD/ld时，总化学需氧量（TCOD）去除效率分别达到88.8 - 72.1%和87.9 - 71.2%。在相同的有机负荷率下比较两个反应器时，发现有载体的反应器总是更高效、更稳定，其TCOD、溶解性化学需氧量（SCOD）去除效率更高，挥发性脂肪酸/碱度比值低于对照反应器。通过对反应器进行质量（TCOD）平衡计算，得出甲烷产率系数Y(G/S)，反应器B和C的该系数分别为0.31和0.30l CH(4)/g TCOD(去除)。通过该平衡计算得到的细胞维持系数k(m)分别为0.0024和0.0036 g TCOD(去除)/g VSSd。对于所研究的两个反应器，体积甲烷产率与可生物降解的TCOD浓度通过米氏方程相关联。该模型对反应器行为的预测非常准确，甲烷产率的实验值和理论值之间的偏差低于10%。
