Kaneko H Hidehiro, Shimada Akiko, Hirayama Kimiaki
Department of Civil and Environmental Engineering, Yamanashi University, 4-3-11 Takeda, Kofu 400-8511, Japan.
Water Res. 2004 Apr;38(8):2173-7. doi: 10.1016/j.watres.2004.02.006.
In order to develop a short-term algal toxicity test, the growth of and the phosphate uptake by the green alga Selenastrum capricornutum during batch culture were observed. In the control medium, S. capricornutum took up phosphate earlier than it grew. It was also observed that the phosphate uptake was inhibited by the presence of a toxicant. From these results, phosphate uptake was considered as one of the useful effect parameters for a short-term algal toxicity test. As the removal rate of phosphate from the medium is a function of the amount of algal cell initially inoculated, the test period is variable. The relationship between the amount of inoculation and phosphate uptake was examined and the test conditions suitable for a 3-h toxicity test were established as one example. According to this test procedure, the inhibitory effect of some toxicants on the phosphate uptake was determined. For comparison, a conventional algal assay based on algal growth was also performed. The EC50s for both tests were close. This indicated that the algal toxicity test method proposed in this paper would be useful for the uses where rapidity is required.
为开发一种短期藻类毒性测试方法，观察了羊角月牙藻（Selenastrum capricornutum）在分批培养过程中的生长情况及其对磷酸盐的吸收。在对照培养基中，羊角月牙藻吸收磷酸盐的时间早于其生长时间。还观察到有毒物质的存在会抑制磷酸盐的吸收。基于这些结果，磷酸盐吸收被认为是短期藻类毒性测试的有用效应参数之一。由于培养基中磷酸盐的去除率是最初接种的藻细胞数量的函数，测试周期是可变的。研究了接种量与磷酸盐吸收之间的关系，并作为一个例子确定了适用于3小时毒性测试的测试条件。根据该测试程序，测定了一些有毒物质对磷酸盐吸收的抑制作用。为作比较，还进行了基于藻类生长的传统藻类测定。两种测试的半数有效浓度（EC50）相近。这表明本文提出的藻类毒性测试方法对于需要快速检测的用途将是有用的。
