Fazekas Zsuzsanna, Gao Dayuan, Saladi Rao N, Lu Yuhun, Lebwohl Mark, Wei Huachen
Department of Dermatology, Mount Sinai School of Medicine, New York, NY 10029, USA.
Nutr Cancer. 2003;47(2):181-7. doi: 10.1207/s15327914nc4702_11.
Lycopene, an acyclic hydrocarbon carotenoid found in tomatoes and their products, is a well-established potent antioxidant, and its anticancer properties have been shown in cultured cells and animal models. We investigated the protective effects of two concentrations of topical lycopene against acute ultraviolet B (UVB)-induced photodamage. Application of lycopene dose dependently inhibited UVB-induced ornithine decarboxylase (P < 0.05) and myeloperoxidase (P < 0.05) and significantly reduced bifold skin thickness (P < 0.05). Immunohistochemical staining revealed increased active caspase-3 of apoptotic pathway in the UVB-exposed group compared with the unexposed control. Application of topical lycopene prevented the cleavage of caspase-3. UVB irradiation completely diminished proliferating cell nuclear antigen (PCNA), and the untreated skin maintained positively stained cells throughout the basal epidermis. Topical application of lycopene significantly reversed UVB-induced PCNA inhibition, and normal PCNA staining was restored in the lycopene-treated skin. Our results suggest that topical lycopene is able to exert its protective effects against acute UVB-induced photodamage. Furthermore, it may act as a preventative agent via inhibition of epidermal ornithine decarboxylase activity, reducing inflammatory responses, maintaining normal cell proliferation, and possibly preventing DNA damage as indicated by blocking the necessitating step of apoptosis following UVB injury.
番茄红素是一种存在于番茄及其制品中的无环烃类类胡萝卜素，是一种公认的强效抗氧化剂，其抗癌特性已在培养细胞和动物模型中得到证实。我们研究了两种浓度的局部应用番茄红素对急性紫外线B（UVB）诱导的光损伤的保护作用。番茄红素的应用呈剂量依赖性地抑制UVB诱导的鸟氨酸脱羧酶（P < 0.05）和髓过氧化物酶（P < 0.05），并显著降低皮肤厚度翻倍（P < 0.05）。免疫组织化学染色显示，与未暴露的对照组相比，UVB暴露组凋亡途径中活化的半胱天冬酶-3增加。局部应用番茄红素可防止半胱天冬酶-3的裂解。UVB照射完全消除了增殖细胞核抗原（PCNA），未处理的皮肤在整个基底表皮中保持阳性染色细胞。局部应用番茄红素可显著逆转UVB诱导的PCNA抑制，并且在番茄红素处理的皮肤中恢复了正常的PCNA染色。我们的结果表明，局部应用番茄红素能够对急性UVB诱导的光损伤发挥保护作用。此外，它可能通过抑制表皮鸟氨酸脱羧酶活性、减少炎症反应、维持正常细胞增殖以及可能通过阻断UVB损伤后凋亡的必要步骤来防止DNA损伤，从而作为一种预防剂发挥作用。
