Copenhaver Christopher C, Gern James E, Li Zhanhai, Shult Peter A, Rosenthal Louis A, Mikus Lance D, Kirk Carol J, Roberg Kathy A, Anderson Elizabeth L, Tisler Christopher J, DaSilva Douglas F, Hiemke Heidi J, Gentile Kevin, Gangnon Ronald E, Lemanske Robert F
Department of Pediatrics, University of Wisconsin Hospital, Madison, WI 53792-9988, USA.
Am J Respir Crit Care Med. 2004 Jul 15;170(2):175-80. doi: 10.1164/rccm.200312-1647OC. Epub 2004 Apr 15.
Daycare attendance and siblings are associated with viral-induced wheezing in children. Preexisting immunologic factors may influence the expression of viral infections in infancy, and in turn, recurrent infections may influence the development of immune responses. A total of 285 children were enrolled in the Childhood Origins of Asthma Project at birth and followed for at least 1 year. Cord blood and 1-year mononuclear cells were stimulated with phytohemagglutinin, and cytokine-response profiles were measured by enzyme-linked immunosorbent assay. Nasal lavage was performed for moderate to severe respiratory illnesses. Daycare attendance and/or siblings significantly increased the likelihood of contracting respiratory syncytial virus (1.5-1.6-fold increase) and rhinovirus (1.8-2.1-fold increase), and increased the risk of rhinovirus-induced wheezing (14-18% vs. 2%, p = 0.011). Cord blood IFN-gamma responses were inversely related to the frequency of viral respiratory infections (r(s) = -0.11, p = 0.05), and more significant for subjects with high exposure to other children (r(s) = -0.27, p = 0.028). The interval change in infantile IFN-gamma responses correlated positively with the frequency of viral infections in infancy (r(s) = 0.12, p = 0.047). These data suggest that neonatal IFN-gamma responses may influence antiviral activity, or may represent a marker of antiviral immunity maturation. Conversely, the frequency of viral infections in infancy can influence IFN-gamma responses.
日托出勤情况和家中兄弟姐妹数量与儿童病毒诱发的喘息有关。先前存在的免疫因素可能会影响婴儿期病毒感染的表现，反过来，反复感染可能会影响免疫反应的发展。共有285名儿童在出生时被纳入哮喘儿童起源项目，并随访至少1年。用植物血凝素刺激脐血和1岁时的单核细胞，通过酶联免疫吸附测定法测量细胞因子反应谱。对中度至重度呼吸道疾病进行鼻腔灌洗。日托出勤情况和/或家中兄弟姐妹数量显著增加了感染呼吸道合胞病毒的可能性（增加1.5至1.6倍）和鼻病毒感染的可能性（增加1.8至2.1倍），并增加了鼻病毒诱发喘息的风险（14%至18%对2%，p = 0.011）。脐血干扰素-γ反应与病毒性呼吸道感染的频率呈负相关（r(s) = -0.11，p = 0.05），对于与其他儿童有高接触的受试者更显著（r(s) = -0.27，p = 0.028）。婴儿期干扰素-γ反应的间隔变化与婴儿期病毒感染的频率呈正相关（r(s) = 0.12，p = 0.047）。这些数据表明，新生儿干扰素-γ反应可能会影响抗病毒活性，或者可能代表抗病毒免疫成熟的一个标志。相反，婴儿期病毒感染的频率可以影响干扰素-γ反应。
