Khobta Andriy, Carlo-Stella Carmelo, Capranico Giovanni
G. Moruzzi Department of Biochemistry, Alma Mater Studiorum University of Bologna, Bologna, Italy.
Cancer Res. 2004 Apr 15;64(8):2656-62. doi: 10.1158/0008-5472.can-03-1126.
The MLL gene breakpoint-cluster region (BCR) is a known hot-spot for chromosomal translocations in human leukemias. We mapped core histone modifications and histone H1 along the MLL gene in Jurkat cells and human CD34(+) progenitor blood cells by chromatin immunoprecipitation. Within the BCR, we found specific histone patterns that were different from other genomic regions and a histone H1-free fragment at the telomeric end. Core histone acetylase/deacetylase activities were also found within the BCR. In the studied cell models, chromatin components at the MLL BCR suggest an asymmetric organization that may influence early molecular events eventually leading to chromosomal translocations.
MLL基因断裂点簇区域（BCR）是人类白血病中已知的染色体易位热点。我们通过染色质免疫沉淀法，在Jurkat细胞和人类CD34(+)祖代血细胞中，绘制了核心组蛋白修饰和组蛋白H1沿MLL基因的分布图。在BCR内，我们发现了与其他基因组区域不同的特定组蛋白模式，以及端粒末端的一个无组蛋白H1片段。在BCR内还发现了核心组蛋白乙酰化酶/去乙酰化酶活性。在研究的细胞模型中，MLL BCR处的染色质成分表明存在一种不对称组织，可能会影响最终导致染色体易位的早期分子事件。
