Novak Predrag, Tepes Predrag, Cindrić Mario, Ilijas Marina, Dragojević Snjezana, Mihaljević Kreso
PLIVA Research Institute Ltd., Prilaz baruna Filipovića 29, HR-10000 Zagreb, Croatia.
J Chromatogr A. 2004 Apr 16;1033(2):299-303. doi: 10.1016/j.chroma.2004.01.055.
Directly coupled LC-MS and LC-NMR were applied to identify and structurally characterize an acarbose degradation product A in acidic media. A comparative analysis of the stop-flow LC-NMR (1H and TOCSY) and LC-MS data provided evidence that A is structurally related to acarbose, differing from the parent compound in a number of subunits present in the molecule. Spectral analysis revealed that A was the alpha-glucosidase inhibitor amylostatin XG. Complementary information obtained from the two methods led to the structural elucidation of A which was later corroborated by high-resolution NMR spectroscopy of the isolated molecule.
