9,11-二顺式-12-氟视紫红质。来自19F-核磁共振研究的发色团特性。

9,11-Dicis-12-fluororhodopsin. Chromophore properties from 19F-NMR studies.

作者信息

Colmenares L U, Liu R S

机构信息

Department of Chemistry, University of Hawaii, Honolulu 96822.

出版信息

Photochem Photobiol. 1992 Jul;56(1):101-5. doi: 10.1111/j.1751-1097.1992.tb09609.x.

DOI:10.1111/j.1751-1097.1992.tb09609.x

PMID:1508977

Abstract

From a 19F-NMR study of 9,11-dicis-12-fluororhodopsin and its photobleached product, we concluded that the initially formed chromophore retained its configuration and the photoproduct corresponded to the two-bond isomerized all-trans. Upon standing, it slowly isomerized to the 9-cis isomer. The method represents a direct, non-destructive procedure for determining configuration purity of the pigment formed. Its unique fluorine opsin shift value is consistent with the expected different orientation of the fluoro-substituent in a dicis pigment.

摘要

通过对9,11-二顺式-12-氟视紫红质及其光漂白产物的19F-核磁共振研究，我们得出结论，最初形成的发色团保留了其构型，光产物对应于双键异构化的全反式。静置时，它会缓慢异构化为9-顺式异构体。该方法是一种用于测定所形成色素构型纯度的直接、无损程序。其独特的氟视蛋白位移值与二顺式色素中氟取代基预期的不同取向一致。