高效毛细管电泳法测定尿中柠檬酸和草酸
[High-performance capillary electrophoresis for determination of urinary citrate and oxalate].
作者信息
Luo Qi-zhi, Dai Kai-jin, Ma An-de, Zhou Jun-ling
机构信息
Central Laboratory, First Military Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2004 Apr;24(4):458-60.
OBJECTIVE
To develop a method for determining urinary citrate and oxalate using high-performance capillary electrophoresis.
METHODS
Capillary electrophoresis with indirect ultraviolet (UV) detection was performed for determining urinary citrate and oxalate. Sodium hromate was used as the UV-absorbing background electrolyte.
RESULTS
Citrate and oxalate had a migration time of 3.76 min and 3.14 min respectively. The calibration curves were linear within a limit of about 2 microg/ml for citrate and about 1 microg/ml for oxalate. The recovery rates of citrate and oxalate were 97.53% and 99.11% respectively.
CONCLUSION
This method is simple, accurate and at low cost for determining citrate and oxalate in the urine.
目的
建立一种利用高效毛细管电泳测定尿中枸橼酸盐和草酸盐的方法。
方法
采用间接紫外检测的毛细管电泳法测定尿中枸橼酸盐和草酸盐。用铬酸钠作为紫外吸收背景电解质。
结果
枸橼酸盐和草酸盐的迁移时间分别为3.76分钟和3.14分钟。枸橼酸盐校准曲线在约2微克/毫升范围内呈线性，草酸盐在约1微克/毫升范围内呈线性。枸橼酸盐和草酸盐的回收率分别为97.53%和99.11%。
结论
该方法用于测定尿中枸橼酸盐和草酸盐简单、准确且成本低。
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