Su Jer Horng, Chang Ming Chung, Lee Yeong Sheng, Tseng I Cheng, Chuang Yin Ching
Department of Biochemistry, Medical College, National Cheng Kung University, Tainan, Taiwan.
Biochim Biophys Acta. 2004 Apr 16;1678(1):7-13. doi: 10.1016/j.bbaexp.2004.01.003.
The gene (lipA) encoding the extracellular lipase and its downstream gene (lipB) from Vibrio vulnificus CKM-1 were cloned and sequenced. Nucleotide sequence analysis and alignments of amino acid sequences suggest that Lip Ais a member of bacterial lipase family I.1 and that LipB is a lipase activator of LipA. The active LipA was produced in recombinant Escherichia coli cells only in the presence of the lipB. In the hydrolysis of p-nitrophenyl esters and triacylglycerols, using the reactivated LipA, the optimum chain lengths for the acyl moiety on the substrate were C14 for ester hydrolysis and C10 to C12 for triacylglycerol hydrolysis.
克隆并测序了创伤弧菌CKM-1编码胞外脂肪酶的基因（lipA）及其下游基因（lipB）。核苷酸序列分析和氨基酸序列比对表明，Lip A是细菌脂肪酶I.1家族的成员，LipB是LipA的脂肪酶激活剂。只有在lipB存在的情况下，活性LipA才能在重组大肠杆菌细胞中产生。使用重新激活的LipA水解对硝基苯酯和三酰甘油时，底物上酰基部分的最佳链长对于酯水解为C14，对于三酰甘油水解为C10至C12。
