P66（ShcA）与丝裂原活化蛋白激酶激活的蛋白激酶2相互作用并调节其活性。
P66(ShcA) interacts with MAPKAP kinase 2 and regulates its activity.
作者信息
Yannoni Yvonne M, Gaestel Matthias, Lin Lih-Ling
机构信息
Department of Inflammation, Wyeth Research, 200 Cambridge Park Drive, Cambridge, MA 02140-2311, USA.
出版信息
FEBS Lett. 2004 Apr 23;564(1-2):205-11. doi: 10.1016/S0014-5793(04)00351-5.
Three mitogen activated protein kinase-activated protein kinase 2 (MAPKAP kinase 2, MK2) interacting proteins were identified using a yeast two-hybrid approach. ShcA, a signaling phospho-protein, human polyhomeotic 2 (HPH2), a transcriptional regulator, and highly similar to smoothelin (HSTS), which is related to the cytoskeletal associated protein smoothelin, interact specifically with MK2. The interaction of MK2 with the 66 kDa isoform of ShcA, p66(ShcA), and HPH2 was confirmed using co-immunoprecipitation. MK2 is activated with p66(ShcA) co-expression and p66(ShcA) is an in vitro substrate for MK2, further demonstrating their association and suggesting a biological role for p66(Shc) in MK2 activation.
利用酵母双杂交方法鉴定出三种丝裂原活化蛋白激酶激活的蛋白激酶2（MAPKAP激酶2，MK2）相互作用蛋白。信号转导磷酸化蛋白ShcA、转录调节因子人类多同源蛋白2（HPH2）以及与细胞骨架相关蛋白平滑肌动蛋白高度相似的HSTS（与平滑肌动蛋白相关），均与MK2特异性相互作用。通过免疫共沉淀证实了MK2与ShcA的66 kDa亚型p66(ShcA)以及HPH2之间的相互作用。MK2在与p66(ShcA)共表达时被激活，并且p66(ShcA)是MK2的体外底物，这进一步证明了它们之间的关联，并暗示了p66(Shc)在MK2激活中的生物学作用。
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