Pasini Evasio, Scarabelli Tiziano M, D'Antona Giuseppe, Dioguardi Francesco S
Service of Metabolic and Nutritional Pathophysiology, Salvatore Maugeri Foundation, IRCCS, Medical Center of Montescano, Pavia, Italy.
Am J Cardiol. 2004 Apr 22;93(8A):30A-34A. doi: 10.1016/j.amjcard.2003.11.007.
Data show that uptake of amino acids correlates with myocardial oxygen consumption after aortic cross-clamp in humans; this suggests a direct link between amino acids and myocardial energy metabolism. The aim of this preliminary study was to investigate the anti-ischemic effects of immediate and long-term supplementation of an amino acid mixture. We tested this hypothesis on isolated rats hearts subjected to global ischemia for 30 minutes. Long-term treatment with an amino acid mixture achieved the following: (1) reduced the increase of diastolic pressure (48 +/- 3 mm Hg vs 21 +/- 4 mm Hg; p <0.05); (2) maintained the tissue content of adenosine triphosphate during ischemia (2.5 +/- 0.6 micromol/g wet wt [gww] vs 7.0 +/- 1.2 micromol/gww; p <0.05); and (3) improved the recovery of developed pressure at the end of postischemic reperfusion (11 +/- 2 mm Hg vs 38 +/- 3 mm Hg; p <0.05), reducing the release of creatine kinase (375 +/- 30 microU/min/gww vs 196 +/- 15 microU/min/gww; p <0.05) and lactate (15 +/- 1.5 mg/min/gww vs 5 +/- 1 mg/min/gww; p <0.05). We conclude that long-term supplementation of an amino acid mixture reduced myocardial ischemic damage.
数据显示，在人体主动脉交叉钳夹后，氨基酸摄取与心肌氧消耗相关；这表明氨基酸与心肌能量代谢之间存在直接联系。这项初步研究的目的是调查立即和长期补充氨基酸混合物的抗缺血作用。我们在经历30分钟全心缺血的离体大鼠心脏上验证了这一假设。长期用氨基酸混合物治疗取得了以下效果：（1）降低舒张压升高幅度（48±3毫米汞柱对21±4毫米汞柱；p<0.05）；（2）在缺血期间维持三磷酸腺苷的组织含量（2.5±0.6微摩尔/克湿重[gww]对7.0±1.2微摩尔/gww；p<0.05）；（3）改善缺血后再灌注结束时的舒张末压恢复情况（11±2毫米汞柱对38±3毫米汞柱；p<0.05），减少肌酸激酶释放（375±30微单位/分钟/gww对196±15微单位/分钟/gww；p<0.05）和乳酸释放（15±1.5毫克/分钟/gww对5±1毫克/分钟/gww；p<0.05）。我们得出结论，长期补充氨基酸混合物可减少心肌缺血损伤。
