Chemin Jean, Nargeot Joël, Lory Philippe
Laboratoire de Génomique Fonctionnelle, UPR 2580 CNRS, Institut de Génétique Humaine, 141 rue de la Cardonille, F-34094 Montpellier cedex 05, France.
Neuroreport. 2004 Mar 22;15(4):671-5. doi: 10.1097/00001756-200403220-00019.
Calcium influx via low-voltage activated alpha(1H) (Ca(v)3.2) T-currents participates in the morphological and electrical differentiation of neuroblastoma NG108-15 cells. We investigated whether an autocrine mechanism could contribute to this differentiation process. The presence of factors secreted by NG108-15 cells was identified through the use of conditioned media (CM) obtained from differentiated cells. These CM significantly increased neuritogenesis with no change in the HVA calcium channel expression. CM-induced neuritogenesis persists during alpha(1H) current block, whereas CM obtained from cells transfected with an alpha(1H) antisense did not induce neuritogenesis. These data indicate that morphological differentiation of NG108-15 cells depends on an autocrine mechanism, which is controlled by alpha(1H) currents. Such a mechanism is likely to play a role in the various differentiation processes that imply alpha(1H) T-type Ca(2+) channels.
通过低电压激活的α(1H)（Ca(v)3.2）T电流介导的钙内流参与神经母细胞瘤NG108-15细胞的形态和电生理分化。我们研究了自分泌机制是否有助于这一分化过程。通过使用从分化细胞获得的条件培养基（CM）来鉴定NG108-15细胞分泌的因子的存在。这些CM显著增加了神经突生成，而高电压激活钙通道表达没有变化。CM诱导的神经突生成在α(1H)电流阻断期间持续存在，而从用α(1H)反义转染的细胞获得的CM则不诱导神经突生成。这些数据表明，NG108-15细胞的形态分化依赖于一种自分泌机制，该机制受α(1H)电流控制。这种机制可能在涉及α(1H) T型Ca(2+)通道的各种分化过程中起作用。
