Hirase Hajime, Qian Lifen, Barthó Peter, Buzsáki György
Center for Molecular and Behavioral Neuroscience, Rutgers University, Newark, New Jersey, USA.
PLoS Biol. 2004 Apr;2(4):E96. doi: 10.1371/journal.pbio.0020096. Epub 2004 Apr 13.
Large and long-lasting cytosolic calcium surges in astrocytes have been described in cultured cells and acute slice preparations. The mechanisms that give rise to these calcium events have been extensively studied in vitro. However, their existence and functions in the intact brain are unknown. We have topically applied Fluo-4 AM on the cerebral cortex of anesthetized rats, and imaged cytosolic calcium fluctuation in astrocyte populations of superficial cortical layers in vivo, using two-photon laser scanning microscopy. Spontaneous Ca(2+) events in individual astrocytes were similar to those observed in vitro. Coordination of Ca(2+) events among astrocytes was indicated by the broad cross-correlograms. Increased neuronal discharge was associated with increased astrocytic Ca(2+) activity in individual cells and a robust coordination of Ca(2+) signals in neighboring astrocytes. These findings indicate potential neuron-glia communication in the intact brain.
在培养细胞和急性脑片制备中，已观察到星形胶质细胞内存在大且持久的胞质钙激增现象。体外已对引发这些钙事件的机制进行了广泛研究。然而，它们在完整大脑中的存在及功能尚不清楚。我们将Fluo-4 AM局部应用于麻醉大鼠的大脑皮层，并使用双光子激光扫描显微镜对体内浅层皮质层星形胶质细胞群体中的胞质钙波动进行成像。单个星形胶质细胞中的自发[Ca(2+)]i事件与体外观察到的相似。宽交叉相关图表明星形胶质细胞之间的[Ca(2+)]i事件存在协同作用。神经元放电增加与单个细胞中星形胶质细胞[Ca(2+)]i活性增加以及相邻星形胶质细胞中[Ca(2+)]i信号的强烈协同作用相关。这些发现表明完整大脑中存在潜在的神经元-胶质细胞通讯。
