Steiner Wes E, Clowers Brian H, English William A, Hill Herbert H
Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA.
Rapid Commun Mass Spectrom. 2004;18(8):882-8. doi: 10.1002/rcm.1419.
The use of an atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) source was employed with an atmospheric pressure ion mobility spectrometer (APIMS) and an orthogonal acceleration reflector time-of-flight mass spectrometer (TOFMS) to analyze dipeptide and biogenic amine mixtures from a liquid glycerol 2,5-dihydroxybenzoic acid (DHB) matrix. Improved sensitivities were obtained by the addition of a localized electrical (corona) discharge in conjunction with the AP-MALDI source. Enhanced sample ionization efficiency created by this combination provided an overall elevation in signal intensity of approximately 1.3 orders in magnitude. Combinations of three dipeptides (Gly-Lys, Ala-Lys, and Val-Lys) and nine biogenic amines (dopamine, serotonin, B-phenylethylamine, tyramine, octopamine, histamine, tryptamine, spermidine, and spermine) were resolved in less than 18 ms. In many cases, reduced mobility constants (K(o)) were determined for these analytes for the first time. Ion mobility drift times, flight times, arbitrary signal intensities, and collision-induced dissociation (CID) fragmentation product signatures are reported for each of the samples.
将大气压基质辅助激光解吸/电离（AP-MALDI）源与大气压离子淌度谱仪（APIMS）和正交加速反射式飞行时间质谱仪（TOFMS）联用，以分析来自液态甘油2,5-二羟基苯甲酸（DHB）基质的二肽和生物胺混合物。通过在AP-MALDI源中加入局部电（电晕）放电，提高了灵敏度。这种组合产生的增强的样品电离效率使信号强度整体提高了约1.3个数量级。三种二肽（甘氨酸-赖氨酸、丙氨酸-赖氨酸和缬氨酸-赖氨酸）和九种生物胺（多巴胺、血清素、β-苯乙胺、酪胺、章鱼胺、组胺、色胺、亚精胺和精胺）的组合在不到18毫秒内得到分离。在许多情况下，首次测定了这些分析物的降低的淌度常数（K(o)）。报告了每个样品的离子淌度漂移时间、飞行时间、任意信号强度和碰撞诱导解离（CID）碎片产物特征。
