Nilsson Mikael, Johansson Gunnar, Isaksson Roland
Department of Chemistry and Biomedical Sciences, University of Kalmar, Kalmar, Sweden.
Electrophoresis. 2004 Apr;25(7-8):1022-7. doi: 10.1002/elps.200305786.
The determination of dissociation constands (K(d)) by competitive ligand binding in partial filling capillary electrophoresis is demonstrated. Two different strategies were applied, one of which only uses a single reporter ligand and a more elaborated one which suppresses systemic disturbances by using a racemic mixture as reporter. The dissociation constants obtained by both alternatives were virtually identical and in good agreement with those previously reported.
通过部分填充毛细管电泳中的竞争性配体结合来测定解离常数（K(d)）已得到证实。应用了两种不同的策略，其中一种仅使用单一报告配体，另一种更精细的策略是使用外消旋混合物作为报告物来抑制系统干扰。两种方法获得的解离常数实际上是相同的，并且与先前报道的结果高度一致。
