Huang Ying, Duan Jianping, Chen Qiaoren, Chen Guonan
Department of Chemistry, Fuzhou University, Fujian, China.
Electrophoresis. 2004 Apr;25(7-8):1051-7. doi: 10.1002/elps.200405810.
Eight enkephalin-related peptides were derivatized using fluorescein isothiocyanate (FITC), and the derivatization products were separated and detected by capillary electrophoresis with laser-induced fluorescence detection. The optimum molar ratio of FITC and peptide for derivatization was found to be 40:1, and 5 mmol/L sodium borate buffer (pH 9.2) was selected as derivatization media in order to get the high efficiency. Enkephalin-related peptides were completely separated in the pH range of 10.51-10.60 in a running buffer consisting of 100 mmol/L sodium borate and 60 mmol/L sodium dodecyl sulfate (SDS). The detection limit for these eight enkephalins ranged from 0.18 to 2.25 nmol/L, and the linear response range was 1.0 x 10(-6) to 1.0 x 10(-9) mol/L with correlation coefficients between 0.9947-0.9988. A separation efficiency as high as 380000 theoretical plates could be obtained for these analytes.
使用异硫氰酸荧光素（FITC）对8种脑啡肽相关肽进行衍生化，衍生化产物通过毛细管电泳-激光诱导荧光检测进行分离和检测。发现FITC与肽衍生化的最佳摩尔比为40:1，为了获得高效率，选择5 mmol/L硼酸钠缓冲液（pH 9.2）作为衍生化介质。在由100 mmol/L硼酸钠和60 mmol/L十二烷基硫酸钠（SDS）组成的运行缓冲液中，脑啡肽相关肽在pH 10.51 - 10.60范围内完全分离。这8种脑啡肽的检测限在0.18至2.25 nmol/L之间，线性响应范围为1.0×10⁻⁶至1.0×10⁻⁹ mol/L，相关系数在0.9947 - 0.9988之间。这些分析物可获得高达380000理论塔板数的分离效率。
