Okumura Yoshiyuki, Ogawa Kenji, Nikai Toshiaki
Department of Quality Control, Mathuurayakugyo Co. Ltd, 24-21 Enjo-chou, Syowa-ku, Nagoya 466-0054, Japan 2Department of Microbiology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tenpaku-ku, Nagoya 468-8503, Japan 3Department of Pulmonary Medicine, National Higashinagoya Hospital, 5-101 Umemorizaka, Meito-ku, Nagoya 465-8620, Japan.
J Med Microbiol. 2004 May;53(Pt 5):351-354. doi: 10.1099/jmm.0.05248-0.
Elastolytic and elastase inhibitory activities were investigated for 13 strains of Aspergillus fumigatus, three strains of Aspergillus flavus and three strains of Aspergillus niger. Nine of the 13 strains of A. fumigatus and all strains of A. flavus demonstrated elastase activity (more than 1 unit ml(-1)). Six of the 13 strains of A. fumigatus and all strains of A. flavus expressed elastase inhibitory activity (more than 2 units ml(-1)). However, no elastase or elastase inhibitory activities were observed with A. niger. It was also found that crude elastase inhibitors from six strains of A. fumigatus and two strains of A. flavus were stable to heat treatment at 100 degrees C for 10 min. In addition, human leukocyte elastases were inhibited by crude elastase inhibitors from A. fumigatus and A. flavus; however, no effect was observed on the elastase derived from porcine pancreas.
对13株烟曲霉、3株黄曲霉和3株黑曲霉的弹性蛋白酶分解活性和弹性蛋白酶抑制活性进行了研究。13株烟曲霉中的9株以及所有黄曲霉菌株均表现出弹性蛋白酶活性（超过1单位/毫升）。13株烟曲霉中的6株以及所有黄曲霉菌株均表现出弹性蛋白酶抑制活性（超过2单位/毫升）。然而，未观察到黑曲霉具有弹性蛋白酶或弹性蛋白酶抑制活性。还发现，来自6株烟曲霉和2株黄曲霉的粗弹性蛋白酶抑制剂在100℃热处理10分钟后仍保持稳定。此外，烟曲霉和黄曲霉的粗弹性蛋白酶抑制剂可抑制人白细胞弹性蛋白酶；然而，对猪胰腺来源的弹性蛋白酶未观察到作用。
Zotero 插件