Hoffer E, Zonis Z, Tabak A, Taitelman U
Israel Poison Information Center, Rambam Medical Center, Haifa.
Vet Hum Toxicol. 1992 Aug;34(4):300-3.
We investigated the effect of desferrioxamine, an effective iron chelator, on animal survival and on plasma vitamin E levels after administration of paraquat doses close to the LD50. Male Sprague-Dawley rats received 20 mg paraquat/kg followed by 300 mg desferrioxamine/kg/d given ip over 2 d at 3 equal intervals. The results suggested that desferrioxamine prevented the paraquat-induced depletion of vitamin E, but did not improve the mortality due to paraquat. In ancillary in vitro experiments with a paraquat-based free radical system, where glutathione reductase and NADPH were used as sources of enzymic activity for the redox cycling of paraquat, desferrioxamine effectively prevented the formation of hydroxyl radicals, as determined by deoxyribose degradation.
我们研究了有效铁螯合剂去铁胺对接近半数致死量百草枯给药后动物存活率及血浆维生素E水平的影响。雄性Sprague-Dawley大鼠接受20 mg/kg百草枯，随后在2天内于3个相等间隔经腹腔注射给予300 mg/kg/d去铁胺。结果表明，去铁胺可防止百草枯诱导的维生素E耗竭，但并未改善百草枯所致的死亡率。在一项以百草枯为基础的自由基体系的辅助体外实验中，使用谷胱甘肽还原酶和NADPH作为百草枯氧化还原循环的酶活性来源，通过脱氧核糖降解测定，去铁胺可有效防止羟自由基的形成。
