Vereecque Rodolphe, Saudemont Aurore, Depil Stéphane, Corm Selim, Andrieux Joris, Soenen-Cornu Valérie, Quesnel Bruno
Unité INSERM 524, Institut de Recherche sur le Cancer de Lille, Lille, France.
Cancer Immunol Immunother. 2004 Sep;53(9):793-8. doi: 10.1007/s00262-004-0528-6. Epub 2004 Apr 20.
Blasts from patients with acute myeloid leukemia (AML) can be differentiated in dendritic cells (DCs) using appropriate combinations of cytokines. However, generation of autologous antileukemic cytotoxic T cells using leukemic DCs remains difficult. We have previously reported that expression of costimulatory molecules in cultured AML cells could be induced by gamma-irradiation. In the present study, blasts from 21 patients with AML were cultured in vitro for 2 days, then cells were gamma-irradiated and antigen-presenting cell (APC) characteristics were assessed. gamma-Irradiation induced expression of several characteristics of APCs in AML blasts, including expression of CD80, CD86, and BDCA-4, and were stimulators of allogeneic mixed lymphocyte reactions. Autologous antileukemic cytotoxicity was induced in seven out of ten cases. This study shows that cells with APC characteristics and able to induce ex vivo stimulation of autologous antileukemic T cells can be generated from AML cells using the simple and rapid method of gamma-irradiation of cultured leukemic cells.
利用细胞因子的适当组合，可将急性髓系白血病（AML）患者的原始细胞分化为树突状细胞（DC）。然而，使用白血病DC生成自体抗白血病细胞毒性T细胞仍然困难。我们之前报道过，γ射线照射可诱导培养的AML细胞共刺激分子的表达。在本研究中，将21例AML患者的原始细胞体外培养2天，然后对细胞进行γ射线照射，并评估抗原呈递细胞（APC）的特征。γ射线照射诱导了AML原始细胞中APC的几个特征的表达，包括CD80、CD86和BDCA-4的表达，并且这些细胞是同种异体混合淋巴细胞反应的刺激物。10例中有7例诱导出了自体抗白血病细胞毒性。本研究表明，使用简单快速的培养白血病细胞γ射线照射方法，可从AML细胞中生成具有APC特征并能在体外诱导自体抗白血病T细胞刺激的细胞。
