Niu Qiao, Niu Piye, He Shuchang
School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
Wei Sheng Yan Jiu. 2004 Jan;33(1):45-8.
In order to investigate the mechanism of the improvement of Gastrodia elata (G.E.) on learning and memory impairment of rats exposed to aluminum.
36 SD rats, adult and healthy, were divided into 6 groups at random: 1. normal saline, 2. Al3+ 5 mg/kg, 3. Al3+ 10 mg/kg, 4. normal saline +G.E., 5. Al3+ 5 mg/kg +G.E. and 6. Al3+ 10 mg/kg +G.E. Then, they were exposed to AlCl3 by intraperitioneal injection at a dose of 0.2 ml/d, 60 d. G.E. was administered by drinking water at a dose of 0.4 g/kg body weight.
The results of learning and memory were recorded by Step-Down Test. It was showed that compared with the normal saline group, in the group of Al3+ 5 mg/kg, the content of brain cortex aluminum and EN1 increased significantly; the latency (LT) was shortened obviously(P < 0.01); the activity of AChE decreased and the activity of MAO increased (P < 0.01). In the group of Al3+ 10 mg/kg, the content of brain cortex aluminum, EN1 and EN2 increased significantly(P < 0.01), the LT is shortened obviously (P < 0.01), the activity of AChE decreased and the activity of MAO increased (P < 0.01). Compared with the group of Al3+ 5 mg/kg, in the group of Al3+ 5 mg/kg +G.E., EN1 decreased significantly (P < 0.05); the LT is prolonged obviously(P < 0.01), the activity of AChE increased(P < 0.05) and the activity of MAO decreased (P < 0.01). Compared with the group of Al3+ 10 mg/kg, in the group of Al3+ 10 mg/kg +G.E., both EN1 and EN2 decreased significantly(P < 0.01); the LT is prolonged obviously(P < 0.01), the activity of AChE increased (P < 0.01) and the activity of MAO decreased (P < 0.01).
It is suggested that G.E. might decrease the content of brain cortex aluminum of rats. However it might improve the learning and memory ability possibly through regulating the cholinergic system and the monoaminergic system.
探讨天麻改善铝暴露大鼠学习记忆障碍的机制。
将36只成年健康SD大鼠随机分为6组：1. 生理盐水组；2. 5mg/kg Al3+组；3. 10mg/kg Al3+组；4. 生理盐水+天麻组；5. 5mg/kg Al3+ +天麻组；6. 10mg/kg Al3+ +天麻组。然后，腹腔注射AlCl3，剂量为0.2ml/d，共60天。天麻通过饮水给药，剂量为0.4g/kg体重。
采用跳台试验记录学习记忆结果。结果显示，与生理盐水组相比，5mg/kg Al3+组大脑皮层铝含量和EN1显著增加；潜伏期明显缩短（P<0.01）；乙酰胆碱酯酶活性降低，单胺氧化酶活性增加（P<0.01）。10mg/kg Al3+组大脑皮层铝、EN1和EN2含量显著增加（P<0.01），潜伏期明显缩短（P<0.01），乙酰胆碱酯酶活性降低，单胺氧化酶活性增加（P<0.01）。与5mg/kg Al3+组相比，5mg/kg Al3+ +天麻组EN1显著降低（P<0.05）；潜伏期明显延长（P<0.01），乙酰胆碱酯酶活性增加（P<0.05），单胺氧化酶活性降低（P<0.01）。与10mg/kg Al3+组相比，10mg/kg Al3+ +天麻组EN1和EN2均显著降低（P<0.01）；潜伏期明显延长（P<0.01），乙酰胆碱酯酶活性增加（P<0.01），单胺氧化酶活性降低（P<0.01）。
提示天麻可能降低大鼠大脑皮层铝含量。但其可能通过调节胆碱能系统和单胺能系统来改善学习记忆能力。
