[蛋白质致敏性的测定——BN大鼠模型]
[Determination of protein allergenicity--BN rat model].
作者信息
Jia Xudong, Li Ning, Wang Wei, Wu Yongning
机构信息
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
出版信息
Wei Sheng Yan Jiu. 2004 Jan;33(1):63-5.
OBJECTIVE
Protein allergenicity was investigated in BN rat model.
METHODS
OVA or BSA was administered i.p. (1 mg/ml) or orally (10 mg/ml) to male BN rats for 6 weeks. In different intervals, blood samples were obtained from the orbital plexus for PCA and histamine determination. In addition, blood pressure of rats in OVA-treated group and control group was measured.
RESULTS
Relatively high titres (1/32-1/128) IgE responses were provoked by administration of OVA, while BSA stimulated only low titres (1/2-1/4) IgE. Compared with the controls, the plasma histamine concentrations of OVA groups were higher. An oral challenge with OVA did not induce a clear effect on blood pressure in the majority of rats. However, two rats demonstrated a temporary drop in blood pressure.
CONCLUSION
BN rat model may be a suitable animal model to determine protein allergenicity.
目的
在BN大鼠模型中研究蛋白质致敏性。
方法
将卵清蛋白（OVA）或牛血清白蛋白（BSA）腹腔注射（1 mg/ml）或口服（10 mg/ml）给雄性BN大鼠，持续6周。在不同时间间隔，从眶静脉丛采集血样用于被动皮肤过敏反应（PCA）和组胺测定。此外，测量OVA处理组和对照组大鼠的血压。
结果
给予OVA可引发相对较高滴度（1/32 - 1/128）的IgE反应，而BSA仅刺激产生低滴度（1/2 - 1/4）的IgE。与对照组相比，OVA组的血浆组胺浓度更高。对大多数大鼠进行OVA口服激发对血压没有明显影响。然而，有两只大鼠出现血压暂时下降。
结论
BN大鼠模型可能是确定蛋白质致敏性的合适动物模型。
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