[Expression and identification of cecropin D gene cloned in Pichia pastoris yeast cells].

OBJECTIVE

Cecropin D gene was cloned into methanol nutritional vector-pPICZ alpha-A, linked with alpha-factor signal peptide.

METHODS

Positive recombinant was gotten through PCR and EcoR I restriction enzyme. The positive recombinant vector was transformed into Pichia pastoris yeast strain GS115.

RESULTS

The positive recombinant yeast velum was fermented and its expressive product was assayed by the method of anti-germ.

CONCLUSION

Cecropin D gene was cloned into yeast cell and its expressive products has high activity of killing bacteria, Which could be developed into a new type of anti-bacterial medicine.