Medial prefrontal cortex and precision of temporal discrimination: a lesion, microinjection, and microdialysis study.

In this paper, we examined the role of the medial prefrontal cortex in temporal discrimination in three experiments using rats. Experiment 1 attempted to dissociate the roles of the medial precentral (PrCm) area from the prelimbic and infralimbic (PL-IL) area in temporal discrimination using fixed-interval (FI) schedule. The gradient of response rate distribution became more moderate by a lesion of the PrCm, but not by a lesion of the PL-IL. In experiment 2, the efflux of acetylcholine (ACh) in the PrCm area during temporal discrimination tasks was compared to that during non-temporal discrimination tasks. ACh efflux was not different between these two tasks. In experiment 3, microinjection of the anticholinergic drug scopolamine (10 microg) into the PrCm area made the gradient of response rate distribution moderate. These findings suggest that reduced activity of the ACh system within the PrCm area impairs the precision of temporal discrimination, even though enhancement of this system is not indispensable for performing temporal discrimination.