Uszyński Mieczysław, Perlik Marek, Uszyński Waldemar, Zekanowska Ewa
Department of Propedeutics of Medicine, Ludwik Rydygier Medical University, Bydgoszcz, Poland.
Eur J Obstet Gynecol Reprod Biol. 2004 May 10;114(1):54-8. doi: 10.1016/j.ejogrb.2003.12.006.
Urokinase plasminogen activator (uPA) and urokinase plasminogen activator receptor (uPAR) are central molecules for uPA/uPAR/plasmin-dependent proteolysis, which is thought to play a significant role in the development of pregnancy, as well as its many complications.
To measure the levels of uPA and uPAR in the placenta and myometrium, as well as in the foetal membranes and amniotic fluid.
The study group consisted of 35 women with normal course of pregnancy, but with complications arising during delivery, which led to Caesarean section. Samples of placenta, myometrium, foetal membranes, amniotic fluid and blood were obtained at the time of operation. Tissue extracts were prepared. Measurements were made by the ELISA method.
uPA and uPAR concentration in gestational tissues, including amniotic fluid, is 100-200 times higher than in plasma. Among tissues, the highest uPA level was found in placenta ( 1.32 +/- 0.48 ng/mg of protein), and the highest uPAR level in foetal membranes (3.33 +/- 1.20 ng/mg of protein).
uPA and uPAR are present in all gestational tissues, in some in relatively high concentrations. Our results support the modern clinical hypothesis that fibrinolytic system can participate in mechanisms of such obstetric complications as pre-term pre-mature rupture of foetal membranes and placental abruption.
尿激酶型纤溶酶原激活剂（uPA）和尿激酶型纤溶酶原激活剂受体（uPAR）是uPA/uPAR/纤溶酶依赖性蛋白水解的核心分子，这一过程被认为在妊娠的发展及其许多并发症中起重要作用。
测量胎盘、子宫肌层、胎膜和羊水中uPA和uPAR的水平。
研究组由35名妊娠过程正常但分娩时出现并发症并导致剖宫产的女性组成。在手术时获取胎盘、子宫肌层、胎膜、羊水和血液样本。制备组织提取物。采用酶联免疫吸附测定（ELISA）法进行测量。
包括羊水在内的妊娠组织中uPA和uPAR的浓度比血浆中高100 - 200倍。在各组织中，胎盘的uPA水平最高（1.32±0.48 ng/mg蛋白质），胎膜的uPAR水平最高（3.33±1.20 ng/mg蛋白质）。
uPA和uPAR存在于所有妊娠组织中，在某些组织中的浓度相对较高。我们的结果支持现代临床假说，即纤溶系统可参与诸如胎膜早破和胎盘早剥等产科并发症的发生机制。
