[微生物法与放射免疫法检测血浆叶酸的比较研究]

[Comparative study of the detection of plasma folate with microbial assay and radioimmunoassay].

作者信息

Hao Ling, Zheng Jun-chi, Tian Yi-hua, Fan Da-wei, Li Zhu

机构信息

Peking University Institute of Reproductive & Child Health, Beijing 100083, China.

出版信息

Beijing Da Xue Xue Bao Yi Xue Ban. 2004 Apr;36(2):210-4.

PMID:15100746

Abstract

OBJECTIVE

To compare two methods (microbial assay and radioimmunoassay) for measuring plasma folate concentrations, and to examine the relationship between plasma folate levels, and alcohol consumption, tobacco use and body mass index, and the risk of hyperhomocysteinemia in China.

METHODS

We used a microtiter plate microbial assay and a radioimmunoassay to measure the folate concentration in 88 plasma samples. After comparing the results of these two methods and fitting a regression line, we examined the geographical, seasonal, and gender differences in folate concentration of plasma collected from 2,422 adults in south and north areas in China, and evaluated the association of plasma folate concentration, with alcohol consumption, cigarette smoking, and body mass index, and with the risk of hyperhomocysteinemia, using the data from the two assays.

RESULTS

The data from the two assays had a linear relationship (r=0.879, P=0.000); the regression was Y=0.683X+0.308 (where X and Y were nature logarithmic transformations of plasma folate by microbial assay and radioimmunoassay, respectively); however, the mean plasma folate levels by microbial assay were much higher than those obtained by radioimmunoassay. Both data sets showed similar plasma folate distributions among Chinese adults, associations with other risk factors, and the risk of hyperhomocysteinemia. We estimated that 19.9% of the Southerners and 67.1% of the Northerners had plasma folate concentrations by radioimmunoassay lower than the 6.8 nmol/L used to define plasma folate deficiency.

CONCLUSION

There is a linear relationship between plasma folate levels determined by microbial assay and radioimmunoassay, but because of the different levels obtained in the two assays, it is difficult to use the microbial assay results to evaluate folate status at this time. The use of 10.5 nmol/L as a cut-off for plasma folate deficiency by microbial assay needs further study.

摘要

目的

比较两种测量血浆叶酸浓度的方法（微生物法和放射免疫法），并研究中国人群血浆叶酸水平与饮酒、吸烟、体重指数以及高同型半胱氨酸血症风险之间的关系。

方法

我们使用微量滴定板微生物法和放射免疫法测量了88份血浆样本中的叶酸浓度。在比较这两种方法的结果并拟合回归线后，我们研究了从中国南北地区2422名成年人采集的血浆中叶酸浓度的地理、季节和性别差异，并使用两种检测的数据评估血浆叶酸浓度与饮酒、吸烟、体重指数以及高同型半胱氨酸血症风险之间的关联。

结果

两种检测的数据呈线性关系（r = 0.879，P = 0.000）；回归方程为Y = 0.683X + 0.308（其中X和Y分别是微生物法和放射免疫法测定的血浆叶酸的自然对数转换值）；然而，微生物法测定的血浆叶酸平均水平远高于放射免疫法。两组数据在中国成年人中显示出相似的血浆叶酸分布、与其他风险因素的关联以及高同型半胱氨酸血症风险。我们估计，通过放射免疫法测定，19.9%的南方人和67.1%的北方人血浆叶酸浓度低于用于定义血浆叶酸缺乏的6.8 nmol/L。