整合宿主因子（IHF）与组蛋白HU：弯曲DNA的灵活架构师

IHF and HU: flexible architects of bent DNA.

作者信息

Swinger Kerren K, Rice Phoebe A

机构信息

Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA.

出版信息

Curr Opin Struct Biol. 2004 Feb;14(1):28-35. doi: 10.1016/j.sbi.2003.12.003.

DOI:10.1016/j.sbi.2003.12.003

PMID:15102446

Abstract

The energetic cost of bending short segments of DNA is very high. This bending is critical for the packaging of DNA and is exploited to regulate many cellular processes. In prokaryotes, IHF and HU are key architectural proteins present at high concentrations. New protein-DNA co-crystal structures, and the adaptation of advanced biophysical and biochemical techniques have led to an improved understanding of how these proteins interact with DNA. These techniques include time-resolved synchrotron X-ray footprinting, differential scanning calorimetry, isothermal titration calorimetry and single-molecule experiments.

摘要

使短片段DNA弯曲的能量消耗非常高。这种弯曲对于DNA的包装至关重要，并被用于调控许多细胞过程。在原核生物中，整合宿主因子（IHF）和类组蛋白（HU）是高浓度存在的关键结构蛋白。新的蛋白质-DNA共晶体结构以及先进生物物理和生化技术的应用，使人们对这些蛋白质如何与DNA相互作用有了更深入的了解。这些技术包括时间分辨同步加速器X射线足迹分析、差示扫描量热法、等温滴定量热法和单分子实验。