α-血红蛋白稳定蛋白基因在人类红细胞生成过程中的表达
Expression of alpha-hemoglobin stabilizing protein gene during human erythropoiesis.
作者信息
dos Santos Camila Oresco, Duarte Adriana S S, Saad Sara Teresinha Olalla, Costa Fernando Ferreira
机构信息
State University of Campinas, Center of Hemotherapy and Hematology, Campinas, São Paulo, Brazil.
出版信息
Exp Hematol. 2004 Feb;32(2):157-62. doi: 10.1016/j.exphem.2003.11.002.
alpha-Hemoglobin stabilizing protein (AHSP) is an abundant, erythroid-specific protein that forms a stable complex with free alpha-hemoglobin but not with beta-hemoglobin or hemoglobin A. As such, AHSP is required for normal erythropoiesis, probably acting by blocking the deleterious effects of free alpha-hemoglobin precipitation. In order to study the levels of expression of the AHSP gene during the different phases of erythropoiesis, we carried out a two-phase liquid culture of erythroid cells and real-time quantitative polymerase chain reaction. Blood from control volunteers was cultured with erythropoietin to stimulate differentiation. The different stages of erythropoiesis were confirmed by morphologic and flow cytometric analysis. The results showed a progressive increase in AHSP gene expression following the expression of alpha-globin gene, during maturation of the red blood cell precursors, confirming the probable important function of this protein during normal erythropoiesis.
α-血红蛋白稳定蛋白（AHSP）是一种丰富的、红细胞特异性蛋白，它与游离的α-血红蛋白形成稳定复合物，但不与β-血红蛋白或血红蛋白A形成复合物。因此，正常红细胞生成需要AHSP，其可能通过阻断游离α-血红蛋白沉淀的有害作用来发挥作用。为了研究红细胞生成不同阶段AHSP基因的表达水平，我们进行了红细胞系细胞的两阶段液体培养和实时定量聚合酶链反应。来自对照志愿者的血液用促红细胞生成素培养以刺激分化。通过形态学和流式细胞术分析确认红细胞生成的不同阶段。结果显示，在红细胞前体成熟过程中，随着α-珠蛋白基因的表达，AHSP基因表达逐渐增加，证实了该蛋白在正常红细胞生成过程中可能具有的重要功能。
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