来自高等植物叶绿体的体外RNA编辑系统。

In vitro RNA editing systems from higher plant chloroplasts.

作者信息

Hirose Tetsuro, Miyamoto Tetsuya, Obokata Junichi, Sugiura Masahiro

机构信息

School of Biomedical Science, Tokyo Medical and Dental University, Japan.

出版信息

Methods Mol Biol. 2004;265:333-44. doi: 10.1385/1-59259-775-0:333.

DOI:10.1385/1-59259-775-0:333

PMID:15103082

Abstract

RNA editing in higher plant chloroplasts involves C-to-U conversion at ~30 specific sites. In vitro systems supporting accurate editing have been developed from tobacco and pea chloroplasts. mRNA substrates labeled with 32P at C residues to be edited provide sensitive detection of editing activity in vitro. The present systems allow the rapid identification of cis-elements using mutated mRNA substrates and trans-acting factors by ultraviolet crosslinking.

摘要

高等植物叶绿体中的RNA编辑涉及约30个特定位点的C到U的转换。已经从烟草和豌豆叶绿体中开发出了支持精确编辑的体外系统。在待编辑的C残基处用32P标记的mRNA底物可在体外灵敏地检测编辑活性。目前的系统允许通过紫外线交联，使用突变的mRNA底物和反式作用因子快速鉴定顺式元件。