生长因子对牛视网膜色素上皮细胞迁移和增殖的影响。
Effect of growth factors on bovine retinal pigment epithelial cell migration and proliferation.
作者信息
Spraul Christoph W, Kaven Corinna, Lang Gerhard K, Lang Gabriele E
机构信息
University Eye Hospital and Clinic of Ulm, Ulm, Germany.
出版信息
Ophthalmic Res. 2004 May-Jun;36(3):166-71. doi: 10.1159/000077330.
BACKGROUND
To examine the effects of platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), acidic fibroblast growth factor (aFGF), insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), and transforming growth factor beta 2 (TGF(beta2)) on bovine retinal pigment epithelial (RPE) cell migration and proliferation.
MATERIALS AND METHODS
Cultured bovine RPE cells were treated with 10 ng/ml PDGF, bFGF, aFGF, IGF-1, EGF, or TGF(beta2). RPE cell migration studies were performed in multiwell plates confluently covered with RPE cells. After inhibition of proliferation and denudation of half of each well, cells were incubated with various growth factors. Migration was measured as the number of cells that had entered the denuded area after 20 h. RPE cell proliferation was determined by [(3)H]thymidine incorporation after growth factor stimulation for 24 h.
RESULTS
RPE cell migration was significantly enhanced after incubation with PDGF (stimulation of 213% compared to the negative control, p = 0.002), bFGF (206%, p = 0.003), aFGF (175%, p = 0.003), IGF-1 (150%, p = 0.003), and EGF (144%, p = 0.003). RPE cell proliferation was stimulated by bFGF (322% compared to the negative control, p < 0.005), PDGF (119%, p < 0.005), aFGF (121%, p < 0.005), and EGF (94%, p < 0.005). IGF-1 showed no significant effect on RPE cell proliferation; TGF(beta2) displayed no effect on RPE cell migration nor on proliferation.
CONCLUSIONS
The peptide growth factors PDGF, bFGF, aFGF, IGF-1, and EGF play an important role in initiating RPE cell migration. Basic FGF, PDGF, aFGF, and EGF stimulate RPE cell DNA synthesis.
背景
研究血小板衍生生长因子（PDGF）、碱性成纤维细胞生长因子（bFGF）、酸性成纤维细胞生长因子（aFGF）、胰岛素样生长因子-1（IGF-1）、表皮生长因子（EGF）和转化生长因子β2（TGF(β2)）对牛视网膜色素上皮（RPE）细胞迁移和增殖的影响。
材料与方法
用10 ng/ml的PDGF、bFGF、aFGF、IGF-1、EGF或TGF(β2)处理培养的牛RPE细胞。RPE细胞迁移研究在铺满RPE细胞的多孔板中进行。在抑制增殖并刮除每个孔的一半细胞后，将细胞与各种生长因子一起孵育。迁移以20小时后进入刮除区域的细胞数量来衡量。在生长因子刺激24小时后，通过[³H]胸腺嘧啶核苷掺入法测定RPE细胞增殖。
结果
与PDGF孵育后，RPE细胞迁移显著增强（与阴性对照相比刺激率为213%，p = 0.002），bFGF（206%，p = 0.003），aFGF（175%，p = 0.003），IGF-1（150%，p = 0.003）和EGF（144%，p = 0.003）。bFGF（与阴性对照相比为322%，p < 0.005）、PDGF（119%，p < 0.005）、aFGF（121%，p < 0.005）和EGF（94%，p < 0.005）刺激RPE细胞增殖。IGF-1对RPE细胞增殖无显著影响；TGF(β2)对RPE细胞迁移和增殖均无影响。
结论
肽生长因子PDGF、bFGF、aFGF、IGF-1和EGF在启动RPE细胞迁移中起重要作用。碱性成纤维细胞生长因子、PDGF、酸性成纤维细胞生长因子和EGF刺激RPE细胞DNA合成。
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