从药用植物冬青叶美登木中分离出的一种阿拉伯半乳聚糖。

An arabinogalactan isolated from the medicinal plant Maytenus ilicifolia.

作者信息

Cipriani Thales R, Mellinger Caroline G, Gorin Philip A J, Iacomini Marcello

机构信息

Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP-19046, CEP-81531-990, Curitiba, PR, Brazil.

出版信息

J Nat Prod. 2004 Apr;67(4):703-6. doi: 10.1021/np034043f.

DOI:10.1021/np034043f

PMID:15104510

Abstract

An arabinogalactan was obtained from the leaves of Maytenus ilicifolia by hot aqueous 2% KOH extraction, followed by a freezing-thawing process and anion-exchange chromatography. It consisted of arabinose, galactose, galacturonic acid, and rhamnose in a 69:20:6:5 molar ratio. Methylation analysis, partial acid hydrolysis, and (13)C NMR spectroscopy indicated that it was an arabinogalactan containing a (1-->4)-linked beta-Galp main chain, substituted at O-6 with Ara units, which were in turn substituted at O-5 (Araf) and/or O-4 (Arap), O-3, O-3,5, and O-2,5. This arabinogalactan is probably linked to O-4 of some Rhap units of a type I rhamnogalacturonan, formed by repeating (1-->4)-alpha-D-GalpA-(1-->2)-alpha-L-Rhap groups.

摘要

通过2%氢氧化钾热水提取、冻融处理和阴离子交换色谱法从冬青叶美登木的叶子中获得了一种阿拉伯半乳聚糖。它由阿拉伯糖、半乳糖、半乳糖醛酸和鼠李糖以69:20:6:5的摩尔比组成。甲基化分析、部分酸水解和(13)C核磁共振光谱表明，它是一种阿拉伯半乳聚糖，含有(1→4)-连接的β-半乳糖主链，在O-6位被阿拉伯糖单元取代，而这些阿拉伯糖单元又在O-5（阿拉伯呋喃糖）和/或O-4（阿拉伯吡喃糖）、O-3、O-3,5和O-2,5位被取代。这种阿拉伯半乳聚糖可能与由重复的(1→4)-α-D-半乳糖醛酸-(1→2)-α-L-鼠李糖基团形成的I型鼠李糖半乳糖醛酸聚糖的一些鼠李糖单元的O-4位相连。