Cipriani Thales R, Mellinger Caroline G, de Souza Lauro M, Baggio Cristiane H, Freitas Cristina S, Marques Maria C A, Gorin Philip A J, Sassaki Guilherme L, Iacomini Marcello
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP-19046, CEP-81531-990, Curitiba, PR, Brazil.
J Nat Prod. 2006 Jul;69(7):1018-21. doi: 10.1021/np060045z.
Maytenus ilicifolia is a medicinal plant used as a tea (infusion) for treatment of stomach ulcers. This tea furnished a polysaccharide after several purification steps, consisting of a freezing-thawing process, Fehling precipitation, ultrafiltration, and dialysis. It consisted of arabinose, galactose, galacturonic acid, 4-O-methylglucuronic acid, rhamnose, and glucose in a 42:41:6:5:4:2 molar ratio. Methylation analysis, controlled Smith degradation, and NMR spectroscopy indicated that it was a type II arabinogalactan containing a (1-->3)-linked beta-d-Galp main chain, substituted at O-6 by (1-->6)-linked beta-d-Galp chains, which were mainly substituted at O-3 by (1-->5)- and (1-->3)-linked alpha-l-Araf chains, and nonreducing end-units of alpha-l-Araf and 4-O-Me-GlcpA. This polysaccharide significantly inhibited ethanol-induced gastric lesions in rats with an ED(50) of 9.3 mg/kg, suggesting that the arabinogalactan liberated from the infusion has a protective anti-ulcer effect.
亮叶美登木是一种药用植物，其制成的茶（浸剂）用于治疗胃溃疡。经过多次纯化步骤，包括冻融过程、费林沉淀、超滤和透析后，这种茶得到了一种多糖。它由阿拉伯糖、半乳糖、半乳糖醛酸、4-O-甲基葡萄糖醛酸、鼠李糖和葡萄糖按42:41:6:5:4:2的摩尔比组成。甲基化分析、可控的史密斯降解和核磁共振光谱表明，它是一种II型阿拉伯半乳聚糖，含有一个(1→3)-连接的β-d-半乳糖主链，在O-6位被(1→6)-连接的β-d-半乳糖链取代，这些链主要在O-3位被(1→5)-和(1→3)-连接的α-l-阿拉伯呋喃糖链取代，以及α-l-阿拉伯呋喃糖和4-O-甲基葡萄糖醛酸的非还原末端单元。这种多糖能显著抑制大鼠乙醇诱导的胃损伤，半数有效剂量（ED50）为9.3毫克/千克，这表明从浸剂中释放出的阿拉伯半乳聚糖具有保护性抗溃疡作用。
