Adelman Miranda K, Schluter Samuel F, Marchalonis John J
Microbiology and Immunology, College of Medicine, University of Arizona, Tucson, AZ 85724, USA.
Protein J. 2004 Feb;23(2):103-18. doi: 10.1023/b:jopc.0000020077.73751.76.
In ancestral sharks, a rapid emergence in the evolution of the immune system occurred, giving jawed-vertebrates the necessary components for the combinatorial immune response (CIR). To compare the natural antibody (NAb) repertoires of the most divergent vertebrates with the capacity to produce antibodies, we isolated NAbs to the same set of antigens by affinity chromatography from two species of Carcharhine sharks and from human polyclonal IgG and IgM antibody preparations. The activities of the affinity-purified anti-T-cell receptor (anti-TCR) NAbs were compared with those of monoclonal anti-TCR NAbs that were generated from a systemic lupus erythematosus patient. We report that sharks and humans, representing the evolutionary extremes of vertebrate species sharing the CIR, have NAbs to human TCRs, Igs, the human senescent cell antigen, and to numerous retroviral antigens, indicating that essential features of the combinatorial repertoire and the capacity to recognize the potential universe of antigens is shared among all jawed-vertebrates.
在远古鲨鱼中，免疫系统在进化过程中迅速出现，为有颌脊椎动物提供了组合免疫反应（CIR）所需的成分。为了比较最具差异的能够产生抗体的脊椎动物的天然抗体（NAb）库，我们通过亲和层析从两种真鲨科鲨鱼以及人多克隆IgG和IgM抗体制剂中分离出针对同一组抗原的NAb。将亲和纯化的抗T细胞受体（anti-TCR）NAb的活性与从一名系统性红斑狼疮患者产生的单克隆抗TCR NAb的活性进行比较。我们报告称，代表共享CIR的脊椎动物进化极端的鲨鱼和人类，具有针对人TCR、Ig、人衰老细胞抗原以及多种逆转录病毒抗原的NAb，这表明组合库的基本特征以及识别潜在抗原宇宙的能力在所有有颌脊椎动物中是共有的。
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