Adyel F Z, Hentati B, Boulila A, Hachicha J, Ternynck T, Avrameas S, Ayadi H
Laboratoire d'Immunologie et de Biologie moléculaire, Faculté de Médecine de Sfax, Tunisia.
J Clin Lab Anal. 1996;10(6):451-7. doi: 10.1002/(SICI)1098-2825(1996)10:6<451::AID-JCLA24>3.0.CO;2-V.
To examine autoantibodies present in patients with active systemic lupus erythematosus (SLE), sera, circulating immune complexes (CIC), and antibodies purified on DNA-immunoadsorbent were tested by enzyme immunoassay. A panel of self-antigens, including DNA, histones (HIS), glomerular basal membrane (GBM), thymus cell extract (TCE), actin (ACT), myosin (MS), and tubulin (TUB), was used to define their specificities. IgM antibodies against all antigens of the panel were detected in sera, CIC, and in antibodies eluted from the DNA-immunoadsorbent and demonstrated a large polyreactivity. IgG antibodies showed restricted activities against DNA, HIS, GBM, and TCE in sera and a large polyreactivity in CIC. Inhibition experiments were performed to assess their mono- or polyreactivities. Among the IgG autoantibody population recognizing DNA, two populations of IgG antibodies were detected in the sera and in the affinity purified anti-DNA: one recognizes DNA, HIS, and GBM, and the other binds to DNA and to cytoskeletal proteins. These autoantibody populations were found in CIC, which also often contained high amounts of IgG antibodies recognizing ACT and MS. A third population of IgG antibody that recognizes only TCE and could not be inhibited by DNA or other antigens was found in serum and CIC. Our data demonstrate the existence of several populations of autoantibody in serum and CIC of SLE patients: (1) IgM polyreactive autoantibodies, (2) IgG polyreactive autoantibodies recognizing DNA and cytoskeletal proteins, (3) IgG specific to DNA, which cross react with HIS and GBM, and (4) IgG specific to TCE antigens.
为检测活动性系统性红斑狼疮（SLE）患者体内存在的自身抗体，采用酶免疫分析法检测血清、循环免疫复合物（CIC）以及在DNA免疫吸附剂上纯化的抗体。使用一组自身抗原，包括DNA、组蛋白（HIS）、肾小球基底膜（GBM）、胸腺细胞提取物（TCE）、肌动蛋白（ACT）、肌球蛋白（MS）和微管蛋白（TUB）来确定其特异性。在血清、CIC以及从DNA免疫吸附剂上洗脱的抗体中均检测到针对该组所有抗原的IgM抗体，并表现出广泛的多反应性。IgG抗体在血清中对DNA、HIS、GBM和TCE表现出有限的活性，而在CIC中表现出广泛的多反应性。进行抑制实验以评估其单反应性或多反应性。在识别DNA的IgG自身抗体群体中，在血清和亲和纯化的抗DNA中检测到两种IgG抗体群体：一种识别DNA、HIS和GBM，另一种与DNA和细胞骨架蛋白结合。这些自身抗体群体存在于CIC中，CIC中也常常含有大量识别ACT和MS的IgG抗体。在血清和CIC中发现了第三种IgG抗体群体，其仅识别TCE，且不能被DNA或其他抗原抑制。我们的数据表明，SLE患者血清和CIC中存在几种自身抗体群体：（1）IgM多反应性自身抗体，（2）识别DNA和细胞骨架蛋白的IgG多反应性自身抗体，（3）与HIS和GBM发生交叉反应的DNA特异性IgG，以及（4）TCE抗原特异性IgG。
