膳食补充剂和生物体液中麻黄碱生物碱及咖啡因浓度的测定。

Determination of ephedra alkaloid and caffeine concentrations in dietary supplements and biological fluids.

作者信息

Jacob Peyton, Haller Christine A, Duan Minjiang, Yu Lisa, Peng Margaret, Benowitz Neal L

机构信息

University of California, San Francisco, Department of Medicine, Division of Clinical Pharmacology, San Francisco General Hospital, 1001 Potrero Avenue, Building 30, 3rd floor, San Francisco, California 94110, USA.

出版信息

J Anal Toxicol. 2004 Apr;28(3):152-9. doi: 10.1093/jat/28.3.152.

DOI:10.1093/jat/28.3.152

PMID:15107144

Abstract

Dietary supplements containing botanical forms of caffeine and ephedra alkaloids have been widely promoted and used in the U.S. for weight loss and athletic enhancement despite a lack of adequate research on the pharmacology of these botanical stimulants. In order to analyze dietary supplements and perform human pharmacokinetic studies, an analytical approach with good precision and accuracy was needed with sufficient sensitivity to detect very low levels of ephedra alkaloids. A liquid chromatography-atmospheric pressure chemical ionization (APCI) tandem mass spectrometry (LC-MS-MS) method was developed for quantitating the various ephedrine-group alkaloids found in dietary supplements that contain Ephedra species, and in plasma and urine of persons consuming these supplements. Using this method, low nanogram-per-milliliter concentrations of ephedrine, pseudoephedrine, norephedrine, norpseudoephedrine, methylephedrine, methylpseudoephedrine, and caffeine can be quantitated in a 12-min LC-MS-MS run.

摘要

尽管对这些植物性兴奋剂的药理学缺乏充分研究，但含有咖啡因和麻黄生物碱植物形式的膳食补充剂在美国已被广泛推广用于减肥和增强运动能力。为了分析膳食补充剂并进行人体药代动力学研究，需要一种具有良好精密度和准确度且灵敏度足以检测极低水平麻黄生物碱的分析方法。开发了一种液相色谱 - 大气压化学电离（APCI）串联质谱（LC-MS-MS）方法，用于定量膳食补充剂中发现的各种麻黄碱类生物碱，这些补充剂含有麻黄属植物，以及食用这些补充剂的人的血浆和尿液中的此类生物碱。使用该方法，在12分钟的LC-MS-MS运行中，可以定量测定低至纳克/毫升浓度的麻黄碱、伪麻黄碱、去甲麻黄碱、去甲伪麻黄碱、甲基麻黄碱、甲基伪麻黄碱和咖啡因。