[Schistosoma japonicum: screening of cDNA library with sera from self-cured water buffaloes (Bos buffelus)].
作者信息
Wang Wen-qin, Liu Shu-xian, Song Guang-cheng, Xu Yu-xin, Cao Jian-ping, Chen Jia-xu
机构信息
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2003;21(5):286-8.
OBJECTIVE
To understand and identify the molecules related to the self-cure of Schistosoma japonicum infection in water buffaloes.
METHODS
The S. japonicum adult worm cDNA library was immunologically screened with the sera of self-cured water buffaloes. The positive clones were identified, cloned, sequenced and analysed with software.
RESULTS
Three genes encoding antigens relevant to sera antibodies in water buffaloes were cloned and sequenced. These antigens included paramyosin (Sj97), GST, carbonyl reductase-like 20-beta-hydroxysteroid dehydrogenase(CR/20-beta-HSD).
CONCLUSION
Sera from self-cured S. japonicum infected water buffaloes can be used to screen adult worm cDNA library for vaccine development.
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