Li Xiao-hong, Liu Shu-xian, Song Guang-cheng, Xu Yu-xin, Cao Jian-ping, Chen Jia-xu
Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2004 Apr 30;22(2):80-2.
To search new potential schistosomiasis vaccine by screening cDNA library with sera of rabbits vaccinated with attenuated larvae.
Schistosoma japonicum (Sj) adult worm cDNA library was screened with sera of rabbits vaccinated with ultraviolet-attenuated schistosomula and by sera of infected rabbits, and the inserts of positive clones were amplified and sequenced.
Six kinds of Sj genes were obtained after three rounds of screening. Among the genes were glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine protease inhibitors (serpin), mitochondrion coding region, part of myosin heavy chain gene and two new genes, respectively.
Screening cDNA library with sera of animals vaccinated with attenuated larvae is an effective way to search new vaccine candidates for schistosomiasis.
通过用减毒幼虫免疫兔的血清筛选cDNA文库，寻找新的潜在血吸虫病疫苗。
用紫外线减毒尾蚴免疫兔的血清以及感染兔的血清筛选日本血吸虫成虫cDNA文库，对阳性克隆的插入片段进行扩增和测序。
经过三轮筛选获得了6种日本血吸虫基因。这些基因分别为甘油醛-3-磷酸脱氢酶（GAPDH）、丝氨酸蛋白酶抑制剂（serpin）、线粒体编码区、部分肌球蛋白重链基因以及两个新基因。
用减毒幼虫免疫动物的血清筛选cDNA文库是寻找血吸虫病新候选疫苗的有效方法。
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