Improved expression of novel red- and green-emitting luciferases of Phrixothrix railroad worms in mammalian cells.
作者信息
Nakajima Yoshihiro, Kimura Takuma, Suzuki Chie, Ohmiya Yoshihiro
机构信息
Cell Dynamics Research Group, Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan.
出版信息
Biosci Biotechnol Biochem. 2004 Apr;68(4):948-51. doi: 10.1271/bbb.68.948.
PMID:15118331
Abstract
Luciferases are widely used for the quantitative monitoring of gene expression in a variety of organisms. We successfully expressed novel red- and green-emitting luciferases of Phrixothrix railroad worms in mammalian cells in combination with the Kozak sequence and the CAG promoter. The characteristic properties of these luciferases indicate that they are appropriate reporter genes for the simultaneous monitoring of two gene expressions.
摘要
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