在疟疾流行地区和非流行地区，通过激光免疫测定法检测新开发的糖磷脂抗原以诊断恶性疟原虫疟疾。

Testing of newly developed glycophospholipid antigen for the detection of P. falciparum malaria by laser light immunoassay in endemic and non-endemic areas.

作者信息

Roy A, Biswas S, Mya M M, Saxena R K, Roy K B

机构信息

Malaria Research Centre (ICMR), 22 Sham Nath Marg, Delhi, India.

出版信息

J Vector Borne Dis. 2003 Sep-Dec;40(3-4):105-8.

PMID:15119080

Abstract

A glycophospholipid (GPL) antigen isolated from Plasmodium falciparum culture supernatant has been tested for its antigenicity. Detection of malaria positive known blood samples and unknown field samples from endemic and non-endemic areas were compared. In this study laser light scattering immunoassay (LIA) was used for the detection of P. falciparum malaria. Test results of control (malaria negative samples from Surat) were compared with known positive samples and unknown malaria positive field samples. A positive correlation has been observed (97%) in falciparum positive samples from laboratory and unknown samples from endemic area (Haldwani) by LIA method using GPL antigen. From the results of the study it was found that GPL antigen has a better antigenic property and can detect almost all the cases of Pf malaria by LIA method.

摘要

从恶性疟原虫培养上清液中分离出的一种糖磷脂（GPL）抗原已对其抗原性进行了检测。对来自流行地区和非流行地区的疟疾阳性已知血样和未知现场样本的检测结果进行了比较。在本研究中，激光散射免疫测定法（LIA）用于检测恶性疟原虫疟疾。将对照（来自苏拉特的疟疾阴性样本）的检测结果与已知阳性样本和未知疟疾阳性现场样本进行了比较。通过使用GPL抗原的LIA方法，在实验室的恶性疟原虫阳性样本与来自流行地区（哈德瓦尼）的未知样本之间观察到了正相关（97%）。从研究结果发现，GPL抗原具有更好的抗原特性，并且通过LIA方法几乎可以检测出所有恶性疟原虫疟疾病例。