RNAIII抑制肽和/或乳酸链球菌素可抑制由甲氧西林敏感和耐甲氧西林表皮葡萄球菌引起的实验性血管移植物感染。

RNAIII-inhibiting peptide and/or nisin inhibit experimental vascular graft infection with methicillin-susceptible and methicillin-resistant Staphylococcus epidermidis.

作者信息

Ghiselli R, Giacometti A, Cirioni O, Dell'Acqua G, Mocchegiani F, Orlando F, D'Amato G, Rocchi M, Scalise G, Saba V

机构信息

Department of General Surgery, INRCA IRRCS, Università Politecnica delle Marche, Ancona, Italy.

出版信息

Eur J Vasc Endovasc Surg. 2004 Jun;27(6):603-7. doi: 10.1016/j.ejvs.2004.03.003.

DOI:10.1016/j.ejvs.2004.03.003

PMID:15121110

Abstract

OBJECTIVE

To investigate the efficacy of RNAIII-inhibiting peptide (RIP) and nisin as prophylactic agents in a rat model of vascular graft infection.

DESIGN

Prospective, randomized, controlled animal study.

MATERIALS

Two hundred and twenty adult male Wistar rats. Staphylococcus epidermidis ATCC 12228 and one clinical isolate of methicillin-resistant S. epidermidis. Drugs: RIP, nisin and rifampin.

METHODS

Graft infections were established in the dorsal subcutaneous tissue by implantation of 1 cm(2) sterile Dacron grafts, followed by topical bacterial inoculation: grafts were retrieved at 7 days. The study included a control group (without inoculation) and two series composed of five groups for each staphylococcal strain: one contaminated group that did not receive any antibiotic prophylaxis, three contaminated groups that received grafts soaked with 10 mg/l RIP, 10 mg/l nisin, 10 mg/l rifampin, or RIP+nisin. The main outcome measure was the extent of bacterial at graft harvest.

RESULTS

The bacterial counts for methicillin-resistant S. epidermidis on explanted grafts were 6.1+/-2.8x10(2), 7.8+/-3.0x10(3) and 5.5+/-2.9x10(4) for RIP, nisin and rifampin, respectively. RIP and nisin used in combination reduced the bacterial count to <10. The results for S. epidermidis were similar.

CONCLUSIONS

RIP and nisin could be used in combination to coat medical devices to prevent drug resistant S. epidermidis infections.

摘要

目的

在大鼠血管移植物感染模型中研究RNAIII抑制肽（RIP）和乳链菌肽作为预防剂的疗效。

设计

前瞻性、随机、对照动物研究。

材料

220只成年雄性Wistar大鼠。表皮葡萄球菌ATCC 12228和1株耐甲氧西林表皮葡萄球菌临床分离株。药物：RIP、乳链菌肽和利福平。

方法

通过植入1 cm²无菌涤纶移植物在背部皮下组织建立移植物感染，随后进行局部细菌接种：7天后取出移植物。该研究包括一个对照组（未接种）和由每种葡萄球菌菌株的五个组组成的两个系列：一个未接受任何抗生素预防的污染组，三个接受用10 mg/l RIP、10 mg/l乳链菌肽、10 mg/l利福平或RIP + 乳链菌肽浸泡的移植物的污染组。主要观察指标是移植物收获时的细菌数量。