Musa Najla B, Abdel Gawad Mahrashan A
Department of Medical Parasitology, Faculty of Medicine, Al Fatah University, Tripoli, Libya.
J Egypt Soc Parasitol. 2004 Apr;34(1):333-44.
Three Babesia divergens isolates have been cultured continuously for 6 months in rat erythrocytes using the candle jar technique. One isolate was already rat-adapted, the other two became adapted to rats through continuous culturing in rat erythrocytes. Babesia was cultured in rat erythrocytes in RPMI medium supplemented with 20% foetal calf serum. The highest parasitaemia was 35% and multi-parasitization of red blood cells was often observed. Cultures of B. divergens remained infective to splenectomized rats. Cultures with high parasitaemias contained a large number of extra-cellular merozoites, when separated from the red blood cells, they retained their infectivity.
使用烛缸技术,三种分歧巴贝斯虫分离株已在大鼠红细胞中连续培养6个月。一种分离株已经适应大鼠,另外两种通过在大鼠红细胞中连续培养而适应大鼠。巴贝斯虫在补充有20%胎牛血清的RPMI培养基中的大鼠红细胞中培养。最高寄生虫血症为35%,并且经常观察到红细胞的多重寄生。分歧巴贝斯虫培养物对脾切除大鼠仍具感染性。具有高寄生虫血症的培养物含有大量细胞外裂殖子,当与红细胞分离时,它们仍保持其感染性。