使用全场光学相干断层扫描的三维细胞水平成像

Three-dimensional cellular-level imaging using full-field optical coherence tomography.

作者信息

Dubois A, Moneron G, Grieve K, Boccara A C

机构信息

Laboratoire d'Optique Physique, Ecole Supérieure de Physique et Chimie Industrielles, Centre National de la Recherche Scientifique, UPR A0005, 10 rue Vauquelin, F-75231 Paris Cedex 5, France.

出版信息

Phys Med Biol. 2004 Apr 7;49(7):1227-34. doi: 10.1088/0031-9155/49/7/010.

DOI:10.1088/0031-9155/49/7/010

PMID:15128200

Abstract

An ultrahigh-resolution full-field optical coherence tomography (OCT) system has been developed for cellular-level imaging of biological media. The system is based on a Linnik interference microscope illuminated with a tungsten halogen lamp, associated with a high-resolution CCD camera. En face tomographic images are produced in real time, with the best spatial resolution ever achieved in OCT (0.7 microm x 0.9 microm, axial x transverse). A shot-noise limited detection sensitivity of 80 dB can be reached with an acquisition time per image of 1 s. Images of animal ophthalmic biopsies and vegetal tissues are shown.

摘要

已开发出一种超高分辨率全场光学相干断层扫描（OCT）系统，用于生物介质的细胞水平成像。该系统基于一台由钨卤灯照明的林尼克干涉显微镜，并配有高分辨率电荷耦合器件（CCD）相机。可实时生成正面断层图像，其空间分辨率在OCT领域达到了前所未有的水平（轴向0.7微米×横向0.9微米）。每幅图像采集时间为1秒时，可实现80分贝的散粒噪声限制检测灵敏度。展示了动物眼科活检组织和植物组织的图像。