Black Jennifer A, Thomson Kenneth S, Pitout Johann D D
Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska, USA.
J Clin Microbiol. 2004 May;42(5):2203-6. doi: 10.1128/JCM.42.5.2203-2206.2004.
Seeking a simple disk test for detection of organisms producing plasmid-mediated AmpC beta-lactamases, we evaluated the diagnostic utility of the beta-lactamase inhibitors 48-1220 (Ro 48-1220) and LN-2-128. Using NCCLS disk methodology, inhibition zone diameters were determined for five beta-lactam antibiotics tested alone and in combination with 20 microg of either 48-1220 or LN-2-128. Using an increase of > or =4 mm in zone diameter in the presence of an inhibitor as a positive test, cefotetan with LN-2-128 and 48-1220 was adequate for the detection of organisms producing plasmid-mediated AmpCs (specificity of 90% and sensitivity of 100%).
为寻找一种用于检测产生质粒介导的AmpCβ-内酰胺酶的微生物的简单纸片试验,我们评估了β-内酰胺酶抑制剂48-1220(Ro 48-1220)和LN-2-128的诊断效用。采用NCCLS纸片法,测定了单独使用以及与20μg的48-1220或LN-2-128联合使用时五种β-内酰胺抗生素的抑菌圈直径。以在抑制剂存在下抑菌圈直径增加≥4mm作为阳性试验,头孢替坦与LN-2-128和48-1220联合使用足以检测出产生质粒介导的AmpC的微生物(特异性为90%,敏感性为100%)。