Beggs Kyle T, Holmes Ann R, Cannon Richard D, Rich Alison M
Department of Oral Sciences, School of Dentistry, University of Otago, Dunedin 9001, New Zealand.
J Clin Microbiol. 2004 May;42(5):2275-8. doi: 10.1128/JCM.42.5.2275-2278.2004.
The feasibility of detecting Candida albicans mRNA in formalin-fixed paraffin-embedded archival human histopathology specimens by reverse transcription-PCR (RT-PCR) was investigated. RT with gene-specific primers was used to detect five single-copy C. albicans gene transcripts, including those of two housekeeping genes, in oral candidiasis samples up to 8 years of age.
研究了通过逆转录聚合酶链反应(RT-PCR)检测福尔马林固定石蜡包埋的存档人类组织病理学标本中白色念珠菌mRNA的可行性。使用基因特异性引物进行逆转录,以检测5种单拷贝白色念珠菌基因转录本,包括2种管家基因的转录本,这些标本来自年龄达8岁的口腔念珠菌病样本。